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Proceedings Paper

Analysis of antifreeze protein activity using colorimetric gold nanosensors
Author(s): Xu Jing; Ho-seok Choi; Ji-In Park; Young-Pil Kim
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Paper Abstract

High activity and long stability of antifreeze proteins (AFPs), also known as ice-binding proteins (IBPs), are necessary for exerting their physiological functions in biotechnology and cryomedicine. Here we report a simple analysis of antifreeze protein activity and stability based on self-assembly of gold nanoparticles (AuNPs) via freezing and thawing cycles. While the mercaptosuccinic acid-capped AuNP (MSA-AuNP) was easily self-assembled after a freezing/thawing cycle, due to the mechanical attack of ice crystal on the MSA-AuNP surface, the presence of AFP impeded the self-assembly of MSA-AuNP via the interaction of AFP with ice crystals via freezing and thawing cycles, which led to a strong color in the MSA-AuNP solution. As a result, the aggregation parameter (E520/E650) of MSA-AuNP showed the rapid detection of both activity and stability of AFPs. We suggest that our newly developed method is very suitable for measuring antifreeze activity and stability in a simple and rapid manner with reliable quantification.

Paper Details

Date Published: 8 July 2015
PDF: 4 pages
Proc. SPIE 9523, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, 95230C (8 July 2015); doi: 10.1117/12.2189039
Show Author Affiliations
Xu Jing, Hanyang Univ. (Korea, Republic of)
Ho-seok Choi, Hanyang Univ. (Korea, Republic of)
Ji-In Park, Hanyang Univ. (Korea, Republic of)
Young-Pil Kim, Hanyang Univ. (Korea, Republic of)


Published in SPIE Proceedings Vol. 9523:
International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015
Donghyun Kim; Min-Gon Kim; Seung-Han Park, Editor(s)

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