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Proceedings Paper

3D nanoscale imaging of biological samples with laboratory-based soft X-ray sources
Author(s): Aurélie Dehlinger; Anne Blechschmidt; Daniel Grötzsch; Robert Jung; Birgit Kanngießer; Christian Seim; Holger Stiel
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Paper Abstract

In microscopy, where the theoretical resolution limit depends on the wavelength of the probing light, radiation in the soft X-ray regime can be used to analyze samples that cannot be resolved with visible light microscopes. In the case of soft X-ray microscopy in the water-window, the energy range of the radiation lies between the absorption edges of carbon (at 284 eV, 4.36 nm) and oxygen (543 eV, 2.34 nm). As a result, carbon-based structures, such as biological samples, posses a strong absorption, whereas e.g. water is more transparent to this radiation. Microscopy in the water-window, therefore, allows the structural investigation of aqueous samples with resolutions of a few tens of nanometers and a penetration depth of up to 10μm. The development of highly brilliant laser-produced plasma-sources has enabled the transfer of Xray microscopy, that was formerly bound to synchrotron sources, to the laboratory, which opens the access of this method to a broader scientific community. The Laboratory Transmission X-ray Microscope at the Berlin Laboratory for innovative X-ray technologies (BLiX) runs with a laser produced nitrogen plasma that emits radiation in the soft X-ray regime. The mentioned high penetration depth can be exploited to analyze biological samples in their natural state and with several projection angles. The obtained tomogram is the key to a more precise and global analysis of samples originating from various fields of life science.

Paper Details

Date Published: 22 September 2015
PDF: 9 pages
Proc. SPIE 9589, X-Ray Lasers and Coherent X-Ray Sources: Development and Applications XI, 95890M (22 September 2015); doi: 10.1117/12.2188324
Show Author Affiliations
Aurélie Dehlinger, Berlin Lab. for Innovative X-Ray Technologies (Germany)
Max-Born-Institut (Germany)
Technische Univ. Berlin (Germany)
Anne Blechschmidt, Berlin Lab. for Innovative X-Ray Technologies (Germany)
Technische Univ. Berlin (Germany)
Daniel Grötzsch, Technische Univ. Berlin (Germany)
Robert Jung, Berlin Lab. for Innovative X-Ray Technologies (Germany)
Max-Born-Institut (Germany)
Birgit Kanngießer, Berlin Lab. for Innovative X-Ray Technologies (Germany)
Technische Univ. Berlin (Germany)
Christian Seim, Berlin Lab. for Innovative X-Ray Technologies (Germany)
Max-Born-Institut (Germany)
Holger Stiel, Berlin Lab. for Innovative X-Ray Technologies (Germany)
Max-Born-Institut (Germany)


Published in SPIE Proceedings Vol. 9589:
X-Ray Lasers and Coherent X-Ray Sources: Development and Applications XI
Annie Klisnick; Carmen S. Menoni, Editor(s)

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