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Proceedings Paper

Multiplexed label-free detection of single proteins
Author(s): Aleksandar Sebesta; Alexandar Weigel; Philipp Kukura
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Paper Abstract

We report multiplexed spectral imaging of plasmon resonance shifts in a simple setup, capable of resolving single protein binding events with a high signal to noise ratio of up to 10 for single Fibronectin (450 kDa) proteins. We directly record 2-dimensional, spectrally dispersed images of multiple gold nanorods. The spectra are corrected via an overlapped particle image that correlates arbitrary particle positions to the reference slit position, allowing us to use the full 2D space of the sensor. Together with the simple experimental implementation, our approach paves the way towards routine and efficient label-free detection down to the single molecule level.

Paper Details

Date Published: 26 August 2015
PDF: 6 pages
Proc. SPIE 9554, Nanoimaging and Nanospectroscopy III, 95541D (26 August 2015); doi: 10.1117/12.2188277
Show Author Affiliations
Aleksandar Sebesta, Univ. of Oxford (United Kingdom)
Alexandar Weigel, Univ. of Oxford (United Kingdom)
Philipp Kukura, Univ. of Oxford (United Kingdom)

Published in SPIE Proceedings Vol. 9554:
Nanoimaging and Nanospectroscopy III
Prabhat Verma; Alexander Egner, Editor(s)

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