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Proceedings Paper

Design and manufacture of angle modulated surface plasmon resonance spectrometer
Author(s): Xinlei Zhou; Ke Chen; Xuefeng Mao; Qingxu Yu; Wei Peng
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Paper Abstract

As an emerging biosensing technology, Surface Plasmon Resonance (SPR) technique, characterized by high sensitivity, label-free detection and real-time monitoring, has been extensively applied in biochemical analysis, environmental monitoring and refractive index measurement. In this paper, an angle modulated SPR spectrometer with high resolution is designed and manufactured. First, according to the modeling and simulation for the SPR spectrometer, several key parameters such as the light source, the thickness of golden film and Cr film are determined. Then, an angle modulated SPR spectrometer system based on 5-layers Kretchmann prism structure is developed for biochemical analysis. System performance is tested after the SPR spectrometer established. We test the power stability of the laser first, which is up to 1.504% (5min). Different concentrations of glycerol are measured to demarcate the system. Then, we measured the deionized water ten times continuously, and a resolution of 1.5×10-5 RIU is achieved. At last, different concentrations of glucose solution are measured, and the resonance angles are used to calculate the refractive index of the glucose solutions, which is more accurate than the result of Abbe refractometer. The relationship between concentration and refractive index is presented by liner fitting.

Paper Details

Date Published: 5 August 2015
PDF: 9 pages
Proc. SPIE 9618, 2015 International Conference on Optical Instruments and Technology: Optical Systems and Modern Optoelectronic Instruments, 96180G (5 August 2015); doi: 10.1117/12.2187645
Show Author Affiliations
Xinlei Zhou, Dalian Univ. of Technology (China)
Ke Chen, Dalian Univ. of Technology (China)
Xuefeng Mao, Dalian Univ. of Technology (China)
Qingxu Yu, Dalian Univ. of Technology (China)
Wei Peng, Dalian Univ. of Technology (China)


Published in SPIE Proceedings Vol. 9618:
2015 International Conference on Optical Instruments and Technology: Optical Systems and Modern Optoelectronic Instruments
Yongtian Wang; Xiaodi Tan; Kimio Tatsuno, Editor(s)

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