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Proceedings Paper

Red blood cell three-dimensional morphometry by quantitative phase microscopy
Author(s): F. Merola; P. Memmolo; L. Miccio; O. Gennari; M. Mugnano; P. A. Netti; P. Ferraro
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Paper Abstract

In humans, healthy mature erythrocytes or Red Blood Cells (RBCs) have globule structure and mostly important they lack a cell nucleus and most organelles, thus RBC is an envelope filled of uniform and transparent liquid. Abnormal RBCs may be fragmented or shaped like teardrops, crescents, needles, or a variety of other forms deviating from their regular ordinary shape. Here we show that seeing an erythrocyte-ensemble as nanolens-array, detection of abnormal cells can be made rapidly and efficiently without recurring to subjective shape analysis of image by the doctor or by sophisticated image processing tools, but rather by exploiting their abnormal shape alterations affecting the lens-focusing properties. Demonstration of how aberrations affect the focusing properties of the RBC is given by Hartmann- Shack approach and Zernike polynomial-fitting, as occurs for wavefront aberration correction in adaptive modern astronomic telescopes. The results show how the concept of biological lens could be addressed for revolutionary integration between photonics and biology and that a fast blood pre-screening can be performed by the proposed approach.

Paper Details

Date Published: 22 June 2015
PDF: 6 pages
Proc. SPIE 9529, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II, 95290K (22 June 2015); doi: 10.1117/12.2186785
Show Author Affiliations
F. Merola, Istituto di Cibernetica "E. Caianiello" (Italy)
P. Memmolo, Istituto di Cibernetica "E. Caianiello" (Italy)
Istituto Italiano di Tecnologia (Italy)
L. Miccio, Istituto di Cibernetica "E. Caianiello" (Italy)
O. Gennari, Istituto di Cibernetica "E. Caianiello" (Italy)
M. Mugnano, Istituto di Cibernetica "E. Caianiello" (Italy)
P. A. Netti, Istituto Italiano di Tecnologia (Italy)
P. Ferraro, Istituto di Cibernetica "E. Caianiello" (Italy)

Published in SPIE Proceedings Vol. 9529:
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II
Pietro Ferraro; Simonetta Grilli; Monika Ritsch-Marte; David Stifter, Editor(s)

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