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Proceedings Paper

Detection of cancerous biological tissue areas by means of infrared absorption and SERS spectroscopy of intercellular fluid
Author(s): M. Velicka; V. Urboniene; J. Ceponkus; M. Pucetaite; F. Jankevicius; V. Sablinskas
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Paper Abstract

We present a novel approach to the detection of cancerous kidney tissue areas by measuring vibrational spectra (IR absorption or SERS) of intercellular fluid taken from the tissue. The method is based on spectral analysis of cancerous and normal tissue areas in order to find specific spectral markers. The samples were prepared by sliding the kidney tissue over a substrate - surface of diamond ATR crystal in case of IR absorption or calcium fluoride optical window in case of SERS. For producing the SERS signal the dried fluid film was covered by silver nanoparticle colloidal solution. In order to suppress fluorescence background the measurements were performed in the NIR spectral region with the excitation wavelength of 1064 nm. The most significant spectral differences – spectral markers - were found in the region between 400 and 1800 cm-1, where spectral bands related to various vibrations of fatty acids, glycolipids and carbohydrates are located. Spectral markers in the IR and SERS spectra are different and the methods can complement each other. Both of them have potential to be used directly during surgery. Additionally, IR absorption spectroscopy in ATR mode can be combined with waveguide probe what makes this method usable in vivo.

Paper Details

Date Published: 21 August 2015
PDF: 8 pages
Proc. SPIE 9550, Biosensing and Nanomedicine VIII, 95500A (21 August 2015); doi: 10.1117/12.2186395
Show Author Affiliations
M. Velicka, Vilnius Univ. (Lithuania)
V. Urboniene, Vilnius Univ. (Lithuania)
J. Ceponkus, Vilnius Univ. (Lithuania)
M. Pucetaite, Vilnius Univ. (Lithuania)
F. Jankevicius, Vilnius Univ. (Lithuania)
V. Sablinskas, Vilnius Univ. (Lithuania)

Published in SPIE Proceedings Vol. 9550:
Biosensing and Nanomedicine VIII
Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi, Editor(s)

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