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Proceedings Paper

Multicolor fluorescence microscopic imaging of cancer cells on the plasmonic chip (Presentation Recording)
Author(s): Keiko Tawa; Chisato Sasakawa; Shohei Yamamura; Izumi Shibata; Masatoshi Kataoka
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Paper Abstract

A plasmonic chip which is a metal coated substrate with grating structure can provide the enhanced fluorescence by the grating-coupled surface plasmon field. In our previous studies, bright epi-fluorescence microscopic imaging of neuron cells and sensitive immunosesnsing have been reported. In this study, two kinds of breast cancer cells, MCF-7 and MDA-MB231, were observed with epi-fluorescence microscope on the plasmonic chip with 2D hole-arrays . They were multicolor stained with 4', 6-diamidino-2-phenylindole (DAPI) and allophycocyanin (APC)-labeled anti-epithelial cell adhesion molecule (EpCAM) antibody. Our plasmonic chip provided the brighter fluorescence images of these cells compared with the glass slide. Even in the cells including few EpCAM, the distribution of EpCAM was clearly observed in the cell membrane. It was found that the plasmonic chip can be one of the powerful tools to detect the marker protein existing around the chip surface even at low concentration.

Paper Details

Date Published: 5 October 2015
PDF: 1 pages
Proc. SPIE 9547, Plasmonics: Metallic Nanostructures and Their Optical Properties XIII, 95470I (5 October 2015); doi: 10.1117/12.2186276
Show Author Affiliations
Keiko Tawa, National Institute of Advanced Industrial Science and Technology (Japan)
Kwansei Gakuin Univ. (Japan)
Chisato Sasakawa, National Institute of Advanced Industrial Science and Technology (Japan)
Shohei Yamamura, National Institute of Advanced Industrial Science and Technology (Japan)
Izumi Shibata, National Institute of Advanced Industrial Science and Technology (Japan)
Masatoshi Kataoka, National Institute of Advanced Industrial Science and Technology (Japan)


Published in SPIE Proceedings Vol. 9547:
Plasmonics: Metallic Nanostructures and Their Optical Properties XIII
Allan D. Boardman; Din Ping Tsai, Editor(s)

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