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Proceedings Paper

A hybrid digital holographic microscopy for biological applications
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Paper Abstract

It will be useful in biological applications if two or more physical parameters are simultaneously measured in a digital holographic microscope. In this paper, we present a hybrid digital holographic microscope that can measure simultaneously three-dimensional (3D) phase and 3D fluorescence distributions. This property has big advantage compared with conventional optical microscopes such as phase contrast microscope and fluorescence microscope. We present an optical setup for measuring both phase and fluorescence images. In the experiments, two objects that are fluorescence beads and egera densa are used. The separation method of phase and fluorescence images is presented.

Paper Details

Date Published: 29 July 2015
PDF: 6 pages
Proc. SPIE 9659, International Conference on Photonics Solutions 2015, 965903 (29 July 2015); doi: 10.1117/12.2185665
Show Author Affiliations
O. Matoba, Kobe Univ. (Japan)
X. Quan, Kobe Univ. (Japan)
P. Xia, Kobe Univ. (Japan)
Y. Awatsuji, Kyoto Insitute of Technology (Japan)

Published in SPIE Proceedings Vol. 9659:
International Conference on Photonics Solutions 2015
Surasak Chiangga; Sarun Sumriddetchkajorn, Editor(s)

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