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Proceedings Paper

Cells characterization in microfluidic flows by small angle light scattering and 3D holographic technique
Author(s): David Dannhauser; Pasquale Memmolo; Domenico Rossi; Francesco Merola; Lisa Miccio; Filippo Causa; Pietro Ferraro; Paolo A. Netti
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Paper Abstract

The Light Scattering Profile (LSP) of an individual cell provides a fast and accurate characterization of its morphological properties. By combining a camera-based small angle light scattering apparatus with a microfluidic-induced particle migration technique, it is possible to characterize cells in microfluidic flows. The scattering profile of an individual cell can be fully characterized by our optimized optical light collection system. Viscoelastic-induced particle migration by polyethylene oxide implemented in a low-cost microfluidic device composed of an alignment section and a measuring section opens the possibility of precise, label-free, individual cell analysis. We have studied living cells in microfluidic flows by our light scattering apparatus and by a Digital Holographic Microscope (DHM) system. Our DHM measurements provided an accurate 3D position tracking even in multiple cell conditions.

Paper Details

Date Published: 22 June 2015
PDF: 6 pages
Proc. SPIE 9529, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II, 95290D (22 June 2015); doi: 10.1117/12.2185113
Show Author Affiliations
David Dannhauser, Istituto Italiano di Tecnologia (Italy)
Pasquale Memmolo, Istituto Italiano di Tecnologia (Italy)
Istituto di Cibernetica "E. Caianiello" (Italy)
Domenico Rossi, Istituto Italiano di Tecnologia (Italy)
Francesco Merola, Istituto di Cibernetica "E. Caianiello" (Italy)
Lisa Miccio, Istituto di Cibernetica "E. Caianiello" (Italy)
Filippo Causa, Univ. degli Studi di Napoli Federico II (Italy)
Pietro Ferraro, Istituto di Cibernetica "E. Caianiello" (Italy)
Paolo A. Netti, Istituto Italiano di Tecnologia (Italy)
Univ. degli Studi di Napoli Federico II (Italy)

Published in SPIE Proceedings Vol. 9529:
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II
Pietro Ferraro; Simonetta Grilli; Monika Ritsch-Marte; David Stifter, Editor(s)

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