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Proceedings Paper

Full 3D morphology of diatoms flowing in a microfluidic channel by digital holographic microscopy
Author(s): Roberto Savoia; Pasquale Memmolo; Francesco Merola; Lisa Miccio; Giuliana D'Ippolito; Angelo Fontana; Pietro Ferraro
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Paper Abstract

In this paper, we present a new approach for three-dimensional reconstruction and biovolume estimation of some species of diatoms. An optofluidic platform, composed by an optical tweezer and holographic modulus, is employed to retrieve several holograms at different angular positions, which are processed by the shape from silhouette algorithm to estimate the 3D shape of the cells.

Paper Details

Date Published: 13 July 2015
PDF: 6 pages
Proc. SPIE 9540, Novel Biophotonics Techniques and Applications III, 95400J (13 July 2015); doi: 10.1117/12.2185016
Show Author Affiliations
Roberto Savoia, Istituto di Scienze Applicate e Sistemi Intelligenti, CNR (Italy)
Pasquale Memmolo, Istituto di Scienze Applicate e Sistemi Intelligenti, CNR (Italy)
Istituto Italiano di Tecnologia (Italy)
Francesco Merola, Istituto di Scienze Applicate e Sistemi Intelligenti, CNR (Italy)
Lisa Miccio, Istituto di Scienze Applicate e Sistemi Intelligenti, CNR (Italy)
Giuliana D'Ippolito, Istituto di Chimica Biomolecolare, CNR (Italy)
Angelo Fontana, Istituto di Chimica Biomolecolare, CNR (Italy)
Pietro Ferraro, Istituto di Scienze Applicate e Sistemi Intelligenti, CNR (Italy)


Published in SPIE Proceedings Vol. 9540:
Novel Biophotonics Techniques and Applications III
Arjen Amelink; I. Alex Vitkin, Editor(s)

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