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Proceedings Paper

Recent developments in light sheet ultramicroscopy imaging techniques
Author(s): Saiedeh Saghafi; Klaus Becker; Nina Jährling; Christian Hahn; Hans-Ulrich Dodt
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Paper Abstract

Ultramicroscopy-UM allows 3D-vizualization of biological specimens with μm-resolution. The spatial intensity distribution and thickness of the light sheet have the foremost impact on the quality of image in UM. In this paper, we present different designs for generating light sheet.

The optical unit in standard UM as well as two optimized designs for generating light sheet are reviewed and discussed. The optical characteristics of their light sheet, length and diameter of the line of focus and spatial intensity distribution along the light sheet, are compared. The experimental results demonstrate a marked improvement in reconstructions of representative biological specimens in the third design utilizing aspheric elements. Furthermore, we present our latest achievements in the clearing process of the specimens as well as detection signals by microscope’s objectives that improve further the image quality.

Paper Details

Date Published: 14 July 2015
PDF: 7 pages
Proc. SPIE 9536, Advanced Microscopy Techniques IV; and Neurophotonics II, 95360C (14 July 2015); doi: 10.1117/12.2184919
Show Author Affiliations
Saiedeh Saghafi, Vienna Univ. of Technology (Austria)
Medical Univ. of Vienna (Austria)
Klaus Becker, Vienna Univ. of Technology (Austria)
Medical Univ. of Vienna (Austria)
Nina Jährling, Vienna Univ. of Technology (Austria)
Medical Univ. of Vienna (Austria)
Christian Hahn, Vienna Univ. of Technology (Austria)
Medical Univ. of Vienna (Austria)
Hans-Ulrich Dodt, Vienna Univ. of Technology (Austria)
Medical Univ. of Vienna (Austria)


Published in SPIE Proceedings Vol. 9536:
Advanced Microscopy Techniques IV; and Neurophotonics II
Emmanuel Beaurepaire; Francesco Pavone; Elizabeth M. Hillman; Peter T. C. So, Editor(s)

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