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Proceedings Paper

Multi-illumination Gabor holography recorded in a single camera snap-shot for high-resolution phase retrieval in digital in-line holographic microscopy
Author(s): Martin Sanz; Jose Angel Picazo-Bueno; Javier Garcia; Vicente Micó
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Paper Abstract

In this contribution we introduce MISHELF microscopy, a new concept and design of a lensless holographic microscope based on wavelength multiplexing, single hologram acquisition and digital image processing. The technique which name comes from Multi-Illumination Single-Holographic-Exposure Lensless Fresnel microscopy, is based on the simultaneous illumination and recording of three diffraction patterns in the Fresnel domain. In combination with a novel and fast iterative phase retrieval algorithm, MISHELF microscopy is capable of high-resolution (micron range) phase-retrieved (twin image elimination) biological imaging of dynamic events (video rate recording speed) since it avoids the time multiplexing needed for the in-line hologram sequence recording when using conventional phase-shifting or phase retrieval algorithms. MISHELF microscopy is validated using two different experimental layouts: one using RGB illumination and detection schemes and another using IRRB as illumination while keeping the RGB color camera as detection device. Preliminary experimental results are provided for both experimental layouts using a synthetic object (USAF resolution test target).

Paper Details

Date Published: 22 June 2015
PDF: 8 pages
Proc. SPIE 9529, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II, 95290B (22 June 2015); doi: 10.1117/12.2184792
Show Author Affiliations
Martin Sanz, Univ. de València (Spain)
Jose Angel Picazo-Bueno, Univ. de València (Spain)
Javier Garcia, Univ. de València (Spain)
Vicente Micó, Univ. de València (Spain)

Published in SPIE Proceedings Vol. 9529:
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II
Pietro Ferraro; Simonetta Grilli; Monika Ritsch-Marte; David Stifter, Editor(s)

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