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Proceedings Paper

Rapid detection of EBOLA VP40 in microchip immunofiltration assay
Author(s): Peter Miethe; Dominik Gary; Nadine Hlawatsch; Anne-Marie Gad
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Paper Abstract

In the spring of 2014, the Ebola virus (EBOV) strain Zaire caused a dramatic outbreak in several regions of West Africa. The RT-PCR and antigen capture diagnostic proved to be effective for detecting EBOV in blood and serum. In this paper, we present data of a rapid antigen capture test for the detection of VP40. The test was performed in a microfluidic chip for immunofiltration analysis. The chip integrates all necessary assay components. The analytical sensitivity of the rapid test was 8 ng/ml for recombinant VP40. In serum and whole blood samples spiked with virus culture material, the detection limit was 2.2 x 102 PFU/ml. The performance data of the rapid test (15 min) are comparable to that of the VP40 laboratory ELISA.

Paper Details

Date Published: 21 May 2015
PDF: 7 pages
Proc. SPIE 9490, Advances in Global Health through Sensing Technologies 2015, 94900H (21 May 2015); doi: 10.1117/12.2184649
Show Author Affiliations
Peter Miethe, fzmb GmbH (Germany)
Dominik Gary, fzmb GmbH (Germany)
Nadine Hlawatsch, microfluidic ChipShop GmbH (Germany)
Anne-Marie Gad, Senova GmbH (Germany)

Published in SPIE Proceedings Vol. 9490:
Advances in Global Health through Sensing Technologies 2015
Šárka O. Southern; Mark A. Mentzer; Virginia E. Wotring, Editor(s)

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