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Proceedings Paper

Quantitative phase imaging by wide field lensless digital holographic microscope
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Paper Abstract

Wide field, lensless microscopes have been developed for telemedicine and for resource limited setting [1]. They are based on in-line digital holography which is capable to provide amplitude and phase information resulting from numerical reconstruction. The phase information enables achieving axial resolution in the nanometer range. Hence, such microscopes provide a powerful tool to determine three-dimensional topologies of microstructures. In this contribution, a compact, low-cost, wide field, lensless microscope is presented, which is capable of providing topological profiles of microstructures in transparent material. Our setup consist only of two main components: a CMOSsensor chip and a laser diode without any need of a pinhole. We use this very simple setup to record holograms of microobjects. A wide field of view of ~24 mm², and a lateral resolution of ~2 μm are achieved. Moreover, amplitude and phase information are obtained from the numerical reconstruction of the holograms using a phase retrieval algorithm together with the angular spectrum propagation method. Topographic information of highly transparent micro-objects is obtained from the phase data. We evaluate our system by recording holograms of lines with different depths written by a focused laser beam. A reliable characterization of laser written microstructures is crucial for their functionality. Our results show that this system is valuable for determination of topological profiles of microstructures in transparent material.

Paper Details

Date Published: 22 June 2015
PDF: 8 pages
Proc. SPIE 9529, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II, 952903 (22 June 2015); doi: 10.1117/12.2184471
Show Author Affiliations
A. Adinda-Ougba, Ruhr-Univ. Bochum (Germany)
N. Koukourakis, Technische Univ. Dresden (Germany)
A. Essaidi, Ruhr-Univ. Bochum (Germany)
N. C. Gerhardt, Ruhr-Univ. Bochum (Germany)
M. R. Hofmann, Ruhr-Univ. Bochum (Germany)

Published in SPIE Proceedings Vol. 9529:
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II
Pietro Ferraro; Simonetta Grilli; Monika Ritsch-Marte; David Stifter, Editor(s)

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