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Proceedings Paper

Absorption and fluorescence spectroscopy on a smartphone
Author(s): Md. Arafat Hossain; John Canning; Kevin Cook; Sandra Ast; Peter J. Rutledge; Abbas Jamalipour
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Paper Abstract

A self-powered smartphone-based field-portable “dual” spectrometer has been developed for both absorption and fluorescence measurements. The smartphone’s existing flash LED has sufficient optical irradiance to undertake absorption measurements within a 3D-printed case containing a low cost nano-imprinted polymer diffraction grating. A UV (λex ~ 370 nm) and VIS (λex ~ 450 nm) LED are wired into the circuit of the flash LED to provide an excitation source for fluorescence measurements. Using a customized app on the smartphone, measurements of absorption and fluorescence spectra are demonstrated using pH-sensitive and Zn2+-responsive probes. Detection over a 300 nm span with 0.42 nm/pixel spectral resolution is demonstrated. Despite the low cost and small size of the portable spectrometer, the results compare well with bench top instruments.

Paper Details

Date Published: 1 July 2015
PDF: 4 pages
Proc. SPIE 9655, Fifth Asia-Pacific Optical Sensors Conference, 96551Z (1 July 2015); doi: 10.1117/12.2184354
Show Author Affiliations
Md. Arafat Hossain, The Univ. of Sydney (Australia)
John Canning, The Univ. of Sydney (Australia)
Kevin Cook, The Univ. of Sydney (Australia)
Sandra Ast, The Univ. of Sydney (Australia)
Peter J. Rutledge, The Univ. of Sydney (Australia)
Abbas Jamalipour, The Univ. of Sydney (Australia)

Published in SPIE Proceedings Vol. 9655:
Fifth Asia-Pacific Optical Sensors Conference
Byoungho Lee; Sang-Bae Lee; Yunjiang Rao, Editor(s)

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