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Proceedings Paper

A versatile new technique to clear mouse and human brain
Author(s): Irene Costantini; Antonino Paolo Di Giovanna; Anna Letizia Allegra Mascaro; Ludovico Silvestri; Marie Caroline Müllenbroich; Leonardo Sacconi; Francesco S. Pavone
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Paper Abstract

Large volumes imaging with microscopic resolution is limited by light scattering. In the last few years based on refractive index matching, different clearing approaches have been developed. Organic solvents and water-based optical clearing agents have been used for optical clearing of entire mouse brain. Although these methods guarantee high transparency and preservation of the fluorescence, though present other non-negligible limitations. Tissue transformation by CLARITY allows high transparency, whole brain immunolabelling and structural and molecular preservation. This method however requires a highly expensive refractive index matching solution limiting practical applicability. In this work we investigate the effectiveness of a water-soluble clearing agent, the 2,2'-thiodiethanol (TDE) to clear mouse and human brain. TDE does not quench the fluorescence signal, is compatible with immunostaining and does not introduce any deformation at sub-cellular level. The not viscous nature of the TDE make it a suitable agent to perform brain slicing during serial two-photon (STP) tomography. In fact, by improving penetration depth it reduces tissue slicing, decreasing the acquisition time and cutting artefacts. TDE can also be used as a refractive index medium for CLARITY. The potential of this method has been explored by imaging a whole transgenic mouse brain with the light sheet microscope. Moreover we apply this technique also on blocks of dysplastic human brain tissue transformed with CLARITY and labeled with different antibody. This clearing approach significantly expands the application of single and two-photon imaging, providing a new useful method for quantitative morphological analysis of structure in mouse and human brain.

Paper Details

Date Published: 14 July 2015
PDF: 6 pages
Proc. SPIE 9536, Advanced Microscopy Techniques IV; and Neurophotonics II, 95360X (14 July 2015); doi: 10.1117/12.2184289
Show Author Affiliations
Irene Costantini, Univ. of Florence (Italy)
Antonino Paolo Di Giovanna, Univ. of Florence (Italy)
Anna Letizia Allegra Mascaro, Univ. of Florence (Italy)
Ludovico Silvestri, Univ. of Florence (Italy)
Marie Caroline Müllenbroich, Univ. of Florence (Italy)
Leonardo Sacconi, National Institute of Optics (Italy)
Univ. of Florence (Italy)
Francesco S. Pavone, Univ. of Florence (Italy)
National Institute of Optics (Italy)

Published in SPIE Proceedings Vol. 9536:
Advanced Microscopy Techniques IV; and Neurophotonics II
Emmanuel Beaurepaire; Francesco Pavone; Elizabeth M. Hillman; Peter T. C. So, Editor(s)

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