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Proceedings Paper

Time-resolved study of microorganisms by Raman spectroscopy
Author(s): Ota Samek; Andrea Haronikova; Stanislav Obruca; Silvie Bernatova; Jan Jezek; Martin Siler; Katarina Mlynarikova; Pavel Zemanek
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Paper Abstract

The main goal of our investigations is to focus on the basic physiological mechanisms of microorganisms (yeast and bacteria), exposed to different conditions, by time-resolved Raman spectroscopy. This study provides an insight into the mechanism of targeted stress factors or the influence of different cultivation times on species metabolism in vivo, in realtime and label free. We also focused on time-course study of physico-chemical properties of bacterial cells and cell cytoplasm with respect to the intracellular content of polyhydroxyalkanoates and to the production of yeast lipids or carotenoids.

Paper Details

Date Published: 15 July 2015
PDF: 5 pages
Proc. SPIE 9537, Clinical and Biomedical Spectroscopy and Imaging IV, 953703 (15 July 2015); doi: 10.1117/12.2183950
Show Author Affiliations
Ota Samek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Andrea Haronikova, Brno Univ. of Technology (Czech Republic)
Stanislav Obruca, Brno Univ. of Technology (Czech Republic)
Silvie Bernatova, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Jan Jezek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Martin Siler, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Katarina Mlynarikova, St. Anne's Faculty Hospital (Czech Republic)
Pavel Zemanek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)


Published in SPIE Proceedings Vol. 9537:
Clinical and Biomedical Spectroscopy and Imaging IV
J. Quincy Brown; Volker Deckert, Editor(s)

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