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Proceedings Paper

Visualization of hemodynamics and light scattering in exposed brain of rat using multispectral image reconstruction based on Wiener estimation method
Author(s): Izumi Nishidate; Tomohiro Ishizuka; Keiichiro Yoshida; Satoko Kawauchi; Shunichi Sato; Manabu Sato
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Paper Abstract

We investigate a method to estimate the spectral images of reduced scattering coefficients and the absorption coefficients of in vivo exposed brain tissues in the range from visible to near-infrared wavelength (500-760 nm) based on diffuse reflectance spectroscopy using a digital RGB camera. In the proposed method, the multi-spectral reflectance images of in vivo exposed brain are reconstructed from the digital red, green, blue images using the Wiener estimation algorithm. The Monte Carlo simulation-based multiple regression analysis for the absorbance spectra is then used to specify the absorption and scattering parameters of brain tissue. In this analysis, the concentration of oxygenated hemoglobin and that of deoxygenated hemoglobin are estimated as the absorption parameters whereas the scattering amplitude a and the scattering power b in the expression of μs’=aλ-b as the scattering parameters, respectively. The spectra of absorption and reduced scattering coefficients are reconstructed from the absorption and scattering parameters, and finally, the spectral images of absorption and reduced scattering coefficients are estimated. We performed simultaneous recordings of spectral diffuse reflectance images and of the electrophysiological signals for in vivo exposed rat brain during the cortical spreading depression evoked by the topical application of KCl. Changes in the total hemoglobin concentration and the tissue oxygen saturation imply the temporary change in cerebral blood flow during CSD. Change in the reduced scattering coefficient was observed before the profound increase in the total hemoglobin concentration, and its occurrence was synchronized with the negative dc shift of the local field potential.

Paper Details

Date Published: 14 July 2015
PDF: 11 pages
Proc. SPIE 9536, Advanced Microscopy Techniques IV; and Neurophotonics II, 95360Z (14 July 2015); doi: 10.1117/12.2183759
Show Author Affiliations
Izumi Nishidate, Tokyo Univ. of Agriculture and Technology (Japan)
Tomohiro Ishizuka, Tokyo Univ. of Agriculture and Technology (Japan)
Keiichiro Yoshida, Tokyo Univ. of Agriculture and Technology (Japan)
Satoko Kawauchi, National Defense Medical College (Japan)
Shunichi Sato, National Defense Medical College (Japan)
Manabu Sato, Yamagata Univ. (Japan)


Published in SPIE Proceedings Vol. 9536:
Advanced Microscopy Techniques IV; and Neurophotonics II
Emmanuel Beaurepaire; Peter T. C. So; Francesco Pavone; Elizabeth M. Hillman, Editor(s)

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