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Proceedings Paper

Optical spectroscopy for stereotactic biopsy of brain tumors
Author(s): Niklas Markwardt; Anna von Berg; Sebastian Fiedler; Marcus Goetz; Neda Haj-Hosseini; Christoph Polzer; Herbert Stepp; Petr Zelenkov; Adrian Rühm
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Paper Abstract

Stereotactic biopsy procedure is performed to obtain a tissue sample for diagnosis purposes. Currently, a fiber-based mechano-optical device for stereotactic biopsies of brain tumors is developed. Two different fluorophores are employed to improve the safety and reliability of this procedure: The fluorescence of intravenously applied indocyanine green (ICG) facilitates the recognition of blood vessels and thus helps minimize the risk of cerebral hemorrhages. 5- aminolevulinic-acid-induced protoporphyrin IX (PpIX) fluorescence is used to localize vital tumor tissue. ICG fluorescence detection using a 2-fiber probe turned out to be an applicable method to recognize blood vessels about 1.5 mm ahead of the fiber tip during a brain tumor biopsy. Moreover, the suitability of two different PpIX excitation wavelengths regarding practical aspects was investigated: While PpIX excitation in the violet region (at 405 nm) allows for higher sensitivity, red excitation (at 633 nm) is noticeably superior with regard to blood layers obscuring the fluorescence signal. Contact measurements on brain simulating agar phantoms demonstrated that a typical blood coverage of the tumor reduces the PpIX signal to about 75% and nearly 0% for 633 nm and 405 nm excitation, respectively. As a result, 633 nm seems to be the wavelength of choice for PpIX-assisted detection of high-grade gliomas in stereotactic biopsy.

Paper Details

Date Published: 15 July 2015
PDF: 8 pages
Proc. SPIE 9542, Medical Laser Applications and Laser-Tissue Interactions VII, 954208 (15 July 2015); doi: 10.1117/12.2183741
Show Author Affiliations
Niklas Markwardt, Klinikum der Univ. München (Germany)
Anna von Berg, Klinikum der Univ. München (Germany)
Sebastian Fiedler, Klinikum der Univ. München (Germany)
Marcus Goetz, MRC Systems GmbH (Germany)
Neda Haj-Hosseini, Linköping Univ. (Sweden)
Christoph Polzer, Klinikum der Univ. München (Germany)
Herbert Stepp, Klinikum der Univ. München (Germany)
Petr Zelenkov, N.N. Burdenko Neurosurgical Institute (Russian Federation)
Adrian Rühm, Klinikum der Univ. München (Germany)

Published in SPIE Proceedings Vol. 9542:
Medical Laser Applications and Laser-Tissue Interactions VII
Lothar D. Lilge; Ronald Sroka, Editor(s)

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