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Proceedings Paper

Tomographic phase microscopy using optical tweezers
Author(s): Mor Habaza; Barak Gilboa; Yael Roichman; Natan T. Shaked
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Paper Abstract

We review our technique for tomographic phase microscopy with optical tweezers [1]. This tomographic phase microscopy approach enables full 3-D refractive-index reconstruction. Tomographic phase microscopy measures quantitatively the 3- D distribution of refractive-index in biological cells. We integrated our external interferometric module with holographic optical tweezers for obtaining quantitative phase maps of biological samples from a wide range of angles. The close-tocommon- path, off-axis interferometric system enables a full-rotation tomographic acquisition of a single cell using holographic optical tweezers for trapping and manipulating with a desired array of traps, while acquiring phase information of a single cell from all different angles and maintaining the native surrounding medium. We experimentally demonstrated two reconstruction algorithms: the filtered back-projection method and the Fourier diffraction method for 3-D refractive index imaging of yeast cells.

Paper Details

Date Published: 14 July 2015
PDF: 6 pages
Proc. SPIE 9536, Advanced Microscopy Techniques IV; and Neurophotonics II, 95360H (14 July 2015); doi: 10.1117/12.2183708
Show Author Affiliations
Mor Habaza, Tel Aviv Univ. (Israel)
Barak Gilboa, Tel Aviv Univ. (Israel)
Yael Roichman, Tel Aviv Univ. (Israel)
Natan T. Shaked, Tel Aviv Univ. (Israel)


Published in SPIE Proceedings Vol. 9536:
Advanced Microscopy Techniques IV; and Neurophotonics II
Emmanuel Beaurepaire; Francesco Pavone; Elizabeth M. Hillman; Peter T. C. So, Editor(s)

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