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Proceedings Paper

Healing process study in murine skin superficial wounds treated with the blue LED photocoagulator EMOLED
Author(s): Francesca Rossi; Riccardo Cicchi; Francesca Tatini; Stefano Bacci; Domenico Alfieri; Gaetano De Siena; Francesco S. Pavone; Roberto Pini
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Paper Abstract

A faster healing process was observed in superficial skin wounds after irradiation with the EMOLED photocoagulator. The instrument consists of a compact handheld photocoagulation device, useful for inducing coagulation in superficial abrasions. In this work we present the results of an in vivo study, in a murine model. Two superficial wounds were produced on the back of 12 mice: one area was left untreated, the other one was treated with EMOLED. Healthy skin was used as a control. The animals were sacrificed 3 hours, 12 hours, 1 day, 6 day after treatment. The treatment effects on back skin was monitored by visual observations, histopathological analysis, immuno-histochemical analysis, and nonlinear microscopic imaging performed at each follow up time, finding no adverse reactions and no thermal damage in both treated areas and surrounding tissues. In addition, a faster healing process, a reduced inflammatory response, a higher collagen content, and a better-recovered skin morphology was evidenced in the treated tissue with respect to the untreated tissue. These morphological features were characterized by means of immuno-histochemical analysis, aimed at imaging fibroblasts and myofibroblasts, and by SHG microscopy, aimed at characterizing collagen organization, demonstrating a fully recovered aspect of dermis as well as a faster neocollagenesis in the treated regions. This study demonstrates that the selective photothermal effect we used for inducing immediate coagulation in superficial wounds is associated to a minimal inflammatory response, which provides reduced recovery times and improved healing process.

Paper Details

Date Published: 15 July 2015
PDF: 6 pages
Proc. SPIE 9542, Medical Laser Applications and Laser-Tissue Interactions VII, 95420F (15 July 2015); doi: 10.1117/12.2183670
Show Author Affiliations
Francesca Rossi, Institute of Applied Physics Nello Carrara, NRC (Italy)
Riccardo Cicchi, National Institute of Optics, NRC (Italy)
European Lab. for Non-linear Spectroscopy (Italy)
Francesca Tatini, European Lab. for Non-linear Spectroscopy (Italy)
Stefano Bacci, Research Unit of Histology and Embriology (Italy)
Domenico Alfieri, Light4Tech Firenze S.r.L. (Italy)
Gaetano De Siena, Institute of Applied Physics Nello Carrara, NRC (Italy)
Francesco S. Pavone, National Institute of Optics, NRC (Italy)
European Lab. for Non-linear Spectroscopy (Italy)
Univ. of Florence (Italy)
Roberto Pini, Institute of Applied Physics Nello Carrara, NRC (Italy)


Published in SPIE Proceedings Vol. 9542:
Medical Laser Applications and Laser-Tissue Interactions VII
Lothar D. Lilge; Ronald Sroka, Editor(s)

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