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Proceedings Paper

Hybrid label-free multiphoton and optoacoustic microscopy (MPOM)
Author(s): Dominik Soliman; George J. Tserevelakis; Murad Omar; Vasilis Ntziachristos
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Paper Abstract

Many biological applications require a simultaneous observation of different anatomical features. However, unless potentially harmful staining of the specimens is employed, individual microscopy techniques do generally not provide multi-contrast capabilities. We present a hybrid microscope integrating optoacoustic microscopy and multiphoton microscopy, including second-harmonic generation, into a single device. This combined multiphoton and optoacoustic microscope (MPOM) offers visualization of a broad range of structures by employing different contrast mechanisms and at the same time enables pure label-free imaging of biological systems. We investigate the relative performance of the two microscopy modalities and demonstrate their multi-contrast abilities through the label-free imaging of a zebrafish larva ex vivo, simultaneously visualizing muscles and pigments. This hybrid microscopy application bears great potential for developmental biology studies, enabling more comprehensive information to be obtained from biological specimens without the necessity of staining.

Paper Details

Date Published: 16 July 2015
PDF: 6 pages
Proc. SPIE 9539, Opto-Acoustic Methods and Applications in Biophotonics II, 953908 (16 July 2015); doi: 10.1117/12.2183619
Show Author Affiliations
Dominik Soliman, Institute for Biological and Medical Imaging, Helmholtz Zentrum München (Germany)
Technische Univ. München (Germany)
George J. Tserevelakis, Foundation for Research and Technology (Greece)
Murad Omar, Institute for Biological and Medical Imaging, Helmholtz Zentrum München (Germany)
Technische Univ. München (Germany)
Vasilis Ntziachristos, Institute for Biological and Medical Imaging, Helmholtz Zentrum München (Germany)
Technische Univ. München (Germany)


Published in SPIE Proceedings Vol. 9539:
Opto-Acoustic Methods and Applications in Biophotonics II
Vasilis Ntziachristos; Roger Zemp, Editor(s)

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