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Proceedings Paper

Wide-field lensfree imaging of tissue slides
Author(s): Sophie Nhu An Morel; Antoine Delon; Pierre Blandin; Thomas Bordy; Olivier Cioni; Lionel Hervé; Catherine Fromentin; Jean-Marc Dinten; Cédric Allier
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Paper Abstract

We developed a new imaging tool that can help pathologists in recording wide-field images of tissue slides. We present a simple cost-effective lens-free imaging method to record 2-4μm resolution wide-field (10 mm2 - 6 cm2) images of stained and unstained tissue slides. To our knowledge, our method is the first technique that enables fast (less than 5 minutes) wide-field lens-free imaging of such dense samples. Multiple holograms are recorded with different wavelength illumination, and a multispectral algorithm is used to retrieve both amplitude and phase. Our method can be used to retrieve images of stained tissue slides. For such absorbing object, the useful information is included in the modulus of the reconstructed complex field. Our method can also be applied to retrieve images of unstained tissue slides, where the useful information is in the retrieved phase. This technique is much cheaper and compact than a conventional microscope and could be made portable. Moreover, it enables wide field unstained tissue slides imaging, which could quickly provide useful information, for example on frozen section biopsies, when a rapid diagnosis is needed during surgery.

Paper Details

Date Published: 14 July 2015
PDF: 5 pages
Proc. SPIE 9536, Advanced Microscopy Techniques IV; and Neurophotonics II, 95360K (14 July 2015); doi: 10.1117/12.2183462
Show Author Affiliations
Sophie Nhu An Morel, CEA-LETI (France)
Univ. Grenoble Alpes (France)
Antoine Delon, LIPHY, CNRS (France)
Univ. Grenoble Alpes (France)
Pierre Blandin, CEA-LETI (France)
Univ. Grenoble Alpes (France)
Thomas Bordy, CEA-LETI (France)
Univ. Grenoble Alpes (France)
Olivier Cioni, CEA-LETI (France)
Univ. Grenoble Alpes (France)
Lionel Hervé, CEA-LETI (France)
Univ. Grenoble Alpes (France)
Catherine Fromentin, Lab. d'Anatomocytopathologie, Ctr. Hospitalier Dr. Schaffner (France)
Jean-Marc Dinten, CEA-LETI (France)
Univ. Grenoble Alpes (France)
Cédric Allier, CEA-LETI (France)
Univ. Grenoble Alpes (France)

Published in SPIE Proceedings Vol. 9536:
Advanced Microscopy Techniques IV; and Neurophotonics II
Emmanuel Beaurepaire; Francesco Pavone; Elizabeth M. Hillman; Peter T. C. So, Editor(s)

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