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Proceedings Paper

Increased metabolic activity detected by FLIM in human breast cancer cells with desmoplastic reaction: a pilot study
Author(s): Rodrigo de Andrade Natal; Vitor Bianchin Pelegati; Caroline Bondarik; Guilherme Rossi Mendonça; Sophie Françoise Derchain; Carmen Passos Lima; Carlos Lenz Cesar; Luís Otávio Sarian; José Vassallo
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Paper Abstract

Introduction: In breast cancer (BC), desmoplastic reaction, assembled primarily by fibroblasts, is associated with unfavorable prognosis, but the reason of this fact remains still unclear. In this context, nonlinear optics microscopy, including Fluorescence Lifetime Imaging Microscopy (FLIM), has provided advancement in cellular metabolism research. In this paper, our purpose is to differentiate BC cells metabolism with or without contact to desmoplastic reaction. Formalin fixed, paraffin embedded samples were used at different points of hematoxylin stained sections. Methodology: Sections from 14 patients with invasive ductal breast carcinoma were analyzed with FLIM methodology to NAD(P)H and FAD fluorescence lifetime on a Confocal Upright LSM780 NLO device (Carl Zeiss AG, Germany). Quantification of the fluorescence lifetime and fluorescence intensity was evaluated by SPC Image software (Becker &Hickl) and ImageJ (NIH), respectively. Optical redox ratio was calculated by dividing the FAD fluorescence intensity by NAD(P)H fluorescence intensity. Data value for FLIM measurements and fluorescence intensities were calculated using Wilcoxon test; p< 0.05 was considered significant. Results: BC cells in contact with desmoplastic reaction presented a significantly lower NAD(P)H and FAD fluorescence lifetime. Furthermore, optical redox ratio was also lower in these tumor cells. Conclusion: Our results suggest that contact of BC cells with desmoplastic reaction increase their metabolic activity, which might explain the adverse prognosis of cases associated with higher peritumoral desmoplastic reaction.

Paper Details

Date Published: 14 July 2015
PDF: 7 pages
Proc. SPIE 9536, Advanced Microscopy Techniques IV; and Neurophotonics II, 95360L (14 July 2015); doi: 10.1117/12.2183442
Show Author Affiliations
Rodrigo de Andrade Natal, State Univ. of Campinas (Brazil)
Vitor Bianchin Pelegati, State Univ. of Campinas (Brazil)
Caroline Bondarik, State Univ. of Campinas (Brazil)
Guilherme Rossi Mendonça, State Univ. of Campinas (Brazil)
Sophie Françoise Derchain, State Univ. of Campinas (Brazil)
Carmen Passos Lima, State Univ. of Campinas (Brazil)
Carlos Lenz Cesar, State Univ. of Campinas (Brazil)
Luís Otávio Sarian, State Univ. of Campinas (Brazil)
José Vassallo, State Univ. of Campinas (Brazil)

Published in SPIE Proceedings Vol. 9536:
Advanced Microscopy Techniques IV; and Neurophotonics II
Emmanuel Beaurepaire; Francesco Pavone; Elizabeth M. Hillman; Peter T. C. So, Editor(s)

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