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Proceedings Paper

Monitoring hemodynamics and oxygenation of the kidney in rats by a combined near-infrared spectroscopy and invasive probe approach
Author(s): Dirk Grosenick; Kathleen Cantow; Karen Arakelyan; Heidrun Wabnitz; Bert Flemming; Angela Skalweit; Mechthild Ladwig; Rainer Macdonald; Thoralf Niendorf; Erdmann Seeliger
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Paper Abstract

We have developed a hybrid approach to investigate the dynamics of perfusion and oxygenation in the kidney of rats under pathophysiologically relevant conditions. Our approach combines near-infrared spectroscopy to quantify hemoglobin concentration and oxygen saturation in the renal cortex, and an invasive probe method for measuring total renal blood flow by an ultrasonic probe, perfusion by laser-Doppler fluxmetry, and tissue oxygen tension via fluorescence quenching. Hemoglobin concentration and oxygen saturation were determined from experimental data by a Monte Carlo model. The hybrid approach was applied to investigate and compare temporal changes during several types of interventions such as arterial and venous occlusions, as well as hyperoxia, hypoxia and hypercapnia induced by different mixtures of the inspired gas. The approach was also applied to study the effects of the x-ray contrast medium iodixanol on the kidney.

Paper Details

Date Published: 16 July 2015
PDF: 5 pages
Proc. SPIE 9538, Diffuse Optical Imaging V, 95380L (16 July 2015); doi: 10.1117/12.2183417
Show Author Affiliations
Dirk Grosenick, Physikalisch-Technische Bundesanstalt (Germany)
Kathleen Cantow, Institut für Vegetative Physiologie, Charité Univ. Berlin (Germany)
Karen Arakelyan, Institut für Vegetative Physiologie, Charité Univ. Berlin (Germany)
Max Delbrueck Ctr. for Molecular Medicine (Germany)
Heidrun Wabnitz, Physikalisch-Technische Bundesanstalt (Germany)
Bert Flemming, Institut für Vegetative Physiologie, Charité Univ. Berlin (Germany)
Angela Skalweit, Institut für Vegetative Physiologie, Charité Univ. Berlin (Germany)
Mechthild Ladwig, Institut für Vegetative Physiologie, Charité Univ. Berlin (Germany)
Rainer Macdonald, Physikalisch-Technische Bundesanstalt (Germany)
Thoralf Niendorf, Max Delbrueck Ctr. for Molecular Medicine (Germany)
Erdmann Seeliger, Institut für Vegetative Physiologie, Charité Univ. Berlin (Germany)


Published in SPIE Proceedings Vol. 9538:
Diffuse Optical Imaging V
Hamid Dehghani; Paola Taroni, Editor(s)

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