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Proceedings Paper

Multiplex detection of pathogen biomarkers in human blood, serum, and saliva using silicon photonic microring resonators
Author(s): I. A. Estrada; R. W. Burlingame; A. P. Wang; K. Chawla; T. Grove; J. Wang; S. O. Southern; M. Iqbal; L. C. Gunn; M. A. Gleeson
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Paper Abstract

Genalyte has developed a multiplex silicon photonic chip diagnostics platform (MaverickTM) for rapid detection of up to 32 biological analytes from a drop of sample in just 10 to 20 minutes. The chips are manufactured with waveguides adjacent to ring resonators, and probed with a continuously variable wavelength laser. A shift in the resonant wavelength as mass binds above the ring resonators is measured and is directly proportional to the amount of bound macromolecules. We present here the ability to multiplex the detection of hemorrhagic fever antigens in whole blood, serum, and saliva in a 16 minute assay. Our proof of concept testing of a multiplex antigencapture chip has the ability to detect Zaire Ebola (ZEBOV) recombinant soluble glycoprotein (rsGP), Marburg virus (MARV) Angola recombinant glycoprotein (rGP) and dengue nonstructural protein I (NS1). In parallel, detection of 2 malaria antigens has proven successful, but has yet to be incorporated into multiplex with the others. Each assay performs with sensitivity ranging from 1.6 ng/ml to 39 ng/ml depending on the antigen detected, and with minimal cross-reactivity.

Paper Details

Date Published: 13 May 2015
PDF: 14 pages
Proc. SPIE 9490, Advances in Global Health through Sensing Technologies 2015, 94900E (13 May 2015); doi: 10.1117/12.2183403
Show Author Affiliations
I. A. Estrada, Genalyte, Inc. (United States)
R. W. Burlingame, Genalyte, Inc. (United States)
A. P. Wang, Genalyte, Inc. (United States)
K. Chawla, Genalyte, Inc. (United States)
T. Grove, Genalyte, Inc. (United States)
J. Wang, Genalyte, Inc. (United States)
S. O. Southern, Gaia Medical Institute (United States)
M. Iqbal, Genalyte, Inc. (United States)
L. C. Gunn, Genalyte, Inc. (United States)
M. A. Gleeson, Genalyte, Inc. (United States)

Published in SPIE Proceedings Vol. 9490:
Advances in Global Health through Sensing Technologies 2015
Šárka O. Southern; Mark A. Mentzer; Virginia E. Wotring, Editor(s)

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