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Proceedings Paper

Raman-based imaging uncovers the effects of alginate hydrogel implants in spinal cord injury
Author(s): Roberta Galli; Sandra Tamosaityte; Maria Koch; Kerim H. Sitoci-Ficici; Robert Later; Ortrud Uckermann; Rudolf Beiermeister; Michael Gelinsky; Gabriele Schackert; Matthias Kirsch; Edmund Koch; Gerald Steiner
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Paper Abstract

The treatment of spinal cord injury by using implants that provide a permissive environment for axonal growth is in the focus of the research for regenerative therapies. Here, Raman-based label-free techniques were applied for the characterization of morphochemical properties of surgically induced spinal cord injury in the rat that received an implant of soft unfunctionalized alginate hydrogel. Raman microspectroscopy followed by chemometrics allowed mapping the different degenerative areas, while multimodal multiphoton microscopy (e.g. the combination of coherent anti-Stokes Raman scattering (CARS), endogenous two-photon fluorescence and second harmonic generation on the same platform) enabled to address the morphochemistry of the tissue at cellular level. The regions of injury, characterized by demyelination and scarring, were retrieved and the distribution of key tissue components was evaluated by Raman mapping. The alginate hydrogel was detected in the lesion up to six months after implantation and had positive effects on the nervous tissue. For instance, multimodal multiphoton microscopy complemented the results of Raman mapping, providing the micromorphology of lipid-rich tissue structures by CARS and enabling to discern lipid-rich regions that contained myelinated axons from degenerative regions characterized by myelin fragmentation and presence of foam cells. These findings demonstrate that Raman-based imaging methods provide useful information for the evaluation of alginate implant effects and have therefore the potential to contribute to new strategies for monitoring degenerative and regenerative processes induced in SCI, thereby improving the effectiveness of therapies.

Paper Details

Date Published: 14 July 2015
PDF: 8 pages
Proc. SPIE 9536, Advanced Microscopy Techniques IV; and Neurophotonics II, 95360Y (14 July 2015); doi: 10.1117/12.2183165
Show Author Affiliations
Roberta Galli, Klinik für Anästhesiologie und Intensivtherapie (Germany)
Sandra Tamosaityte, Klinik für Anästhesiologie und Intensivtherapie (Germany)
Maria Koch, Univ. Carl Gustav Carus (Germany)
Ctr. for Regenerative Therapies Dresden (Germany)
Kerim H. Sitoci-Ficici, Univ. Carl Gustav Carus (Germany)
Robert Later, Univ. Carl Gustav Carus (Germany)
Ortrud Uckermann, Univ. Carl Gustav Carus (Germany)
Rudolf Beiermeister, Technische Univ. Dresden (Germany)
Michael Gelinsky, Technische Univ. Dresden (Germany)
Gabriele Schackert, Univ. Carl Gustav Carus (Germany)
Matthias Kirsch, Univ. Carl Gustav Carus (Germany)
Ctr. for Regenerative Therapies Dresden (Germany)
Edmund Koch, Klinik für Anästhesiologie und Intensivtherapie (Germany)
Gerald Steiner, Klinik für Anästhesiologie und Intensivtherapie (Germany)

Published in SPIE Proceedings Vol. 9536:
Advanced Microscopy Techniques IV; and Neurophotonics II
Emmanuel Beaurepaire; Francesco Pavone; Elizabeth M. Hillman; Peter T. C. So, Editor(s)

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