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Proceedings Paper

Confocal scanning microscopy under ultrashort pulse illumination
Author(s): Min Gu; T. Tannous; Colin J. R. Sheppard
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Paper Abstract

We, in this paper, investigate resolution in various confocal imaging methods based on the use of an ultrashort pulsed laser beam. For bright-field confocal microscopy, time-resolved and time averaged imaging methods are considered, while resolution in two-photon and single- photon confocal fluorescence microscopy is examined. It is shown that the time-resolved approach can give a 3D image of a thick object with axial resolution approximately 30% higher than the limiting value under CW laser illumination of the same wavelength as the pulsed beam, while the transverse resolution is slightly improved.

Paper Details

Date Published: 1 September 1995
PDF: 7 pages
Proc. SPIE 2549, Ultrahigh- and High-Speed Photography, Videography, and Photonics '95, (1 September 1995); doi: 10.1117/12.218311
Show Author Affiliations
Min Gu, Univ. of Sydney (Australia)
T. Tannous, Univ. of Sydney (Australia)
Colin J. R. Sheppard, Univ. of Sydney (Australia)

Published in SPIE Proceedings Vol. 2549:
Ultrahigh- and High-Speed Photography, Videography, and Photonics '95
George A. Kyrala; Donald R. Snyder, Editor(s)

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