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Proceedings Paper

Ultra-sensitive detection of biomarker using localized surface plasmon resonance (LSPR) enhanced by ELISA
Author(s): Yong-Beom Shin; Na rae Jo; Ki joong Lee
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Paper Abstract

We demonstrate a highly sensitive detection of AFP (α-fetoprotein) protein (liver cancer marker) in human serum using the LSPR biosensor. Gold metal nanodot array (MNA) on a glass wafer were fabricated by UV nanoimprint lithography (NIL). After the NIL process using a film stamp and the removal of residual layer via oxygen plasma etching, metal films were deposited using an electron-beam evaporator, followed by the lift-off step. Consequently, the gold MNA was realized on 5-inch glass wafer and the pitch, diameter and height of MNA were 300nm, 150 nm and 20 nm, respectively. We employed observation of LSPR spectra via back-reflection, which provides a stable measurement of LSPR because a probe light does not pass a bio-sample. In addition, one channel among two flow channels was used a control channel, the MNA surface in which was modified with bovine serum albumin, not antibody. After antigen-antibody reaction, the enzyme/precipitation was employed on the MNA (Nano-ELISA). As a result, we could detect AFP in 50L human serum with limit of detection (LOD) of 0.7 zeptomole (10-21 mole).

Paper Details

Date Published: 15 July 2015
PDF: 9 pages
Proc. SPIE 9537, Clinical and Biomedical Spectroscopy and Imaging IV, 95371F (15 July 2015); doi: 10.1117/12.2183063
Show Author Affiliations
Yong-Beom Shin, Korea Univ. of Science and Technology (Korea, Republic of)
Korea Research Institute of Bioscience and Biotechnology (Korea, Republic of)
Na rae Jo, Korea Univ. of Science and Technology (Korea, Republic of)
Ki joong Lee, Korea Research Institute of Bioscience and Biotechnology (Korea, Republic of)


Published in SPIE Proceedings Vol. 9537:
Clinical and Biomedical Spectroscopy and Imaging IV
J. Quincy Brown; Volker Deckert, Editor(s)

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