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Proceedings Paper

Opto-fluidic flow analysis for monitoring of immunity levels
Author(s): A. Mohan; A, Bharadwaj; N. Marshkole; T. Saiyed; A. Prabhakar
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Paper Abstract

We describes the design, development and testing of a cost effective and miniaturized version of a flow analyzer. It is designed to detect fluorescence labeled immunocytes in human blood sample. Availing of advancements in micro fluidics and nanolithographic technique, we fabricated a PDMS based device with integrated micro channels for accommodating the optical fibers. The lensed fibers serves as the waveguides for illumination and collection of laser and scattered signals respectively. As a cell crosses the interrogation point the forward scatter, side scatter and fluorescence are detected. Photomultiplier tubes used in conventional flow cytometers have been replaced here with APDs (avalanche photo detectors) and supplemented with digital signal processing. The prototype was tested with different sized polymer beads as well as the live cells.

Paper Details

Date Published: 15 June 2015
PDF: 8 pages
Proc. SPIE 9654, International Conference on Optics and Photonics 2015, 96540W (15 June 2015); doi: 10.1117/12.2182901
Show Author Affiliations
A. Mohan, Indian Institute of Technology Madras (India)
Ctr. for Cellular and Molecular Platforms (India)
A, Bharadwaj, Indian Institute of Technology Madras (India)
Ctr. for Cellular and Molecular Platforms (India)
N. Marshkole, Ctr. for Cellular and Molecular Platforms (India)
T. Saiyed, Ctr. for Cellular and Molecular Platforms (India)
A. Prabhakar, Indian Institute of Technology Madras (India)


Published in SPIE Proceedings Vol. 9654:
International Conference on Optics and Photonics 2015
Kallol Bhattacharya, Editor(s)

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