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Proceedings Paper

On-chip surface-enhanced Raman spectroscopy (SERS)-linked immuno-sensor assay (SLISA) for rapid environmental-surveillance of chemical toxins
Author(s): Vinay Bhardwaj; Supriya Srinivasan; Anthony J. McGoron
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Paper Abstract

The increasing threat of an intentional (attack) or accidental release of toxins, in particular chemical toxins, including chemical warfare agents (CWAs) and toxic industrial chemicals (TICs) has increased public fear. The major problem in such attacks/accidents is to detect toxins present in very low levels. Indeed, several detection techniques are currently being used for the same. However, none of them meet the most critical requirements of a RISE (Rapid, Inexpensive, Simple and Effective) detect-to-protect class of biosensors. To address this critical demand our group has developed a prototype lab-on-a-chip (LOC) using a colloidal silver-based, surface-enhanced Raman spectroscopy (SERS)-linked immuno-sensor assay (SLISA). The LOC-SLISA was tested for the measurement of RAD54, a stress-marker protein expressed by yeast in response to hydrogen peroxide (H2O2), a toxin in the EPA priority list of chemical toxins. We found SLISA has good correlation in accuracy with the traditional ELISA technique and outperforms the latter by being rapid and easy-to-use. SLISA is more sensitive, provides qualitative information on immuno-sensor’s chemical characterization and antigen-antibody binding, and allows direct detection with minimal or no chance of uncertainty, which is a stringent limitation of all label-based biosensor technologies including ELISA. For translational significance of our work, we correlated our results to U.S. EPA (environmental protection agency) defined risk exposure guideline levels of H2O2 to validate the commercial potential of our on-chip SLISA. The label-free, cell-based and RISE detection offered by SERS can allow development of biomedical and environmental sensor technology (BEST) needed for direct, rapid and continuous monitoring of human health and environment

Paper Details

Date Published: 13 May 2015
PDF: 8 pages
Proc. SPIE 9486, Advanced Environmental, Chemical, and Biological Sensing Technologies XII, 948611 (13 May 2015); doi: 10.1117/12.2182591
Show Author Affiliations
Vinay Bhardwaj, Florida International Univ. (United States)
Supriya Srinivasan, Florida International Univ. (United States)
Anthony J. McGoron, Florida International Univ. (United States)

Published in SPIE Proceedings Vol. 9486:
Advanced Environmental, Chemical, and Biological Sensing Technologies XII
Tuan Vo-Dinh; Robert A. Lieberman; Günter G. Gauglitz, Editor(s)

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