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Proceedings Paper

Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response
Author(s): D. G. Waugh; J. Lawrence; P. Shukla; C. Chan; I. Hussain; H. C. Man; G. C. Smith
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Paper Abstract

Mesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO2 laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work.

Paper Details

Date Published: 1 July 2015
PDF: 11 pages
Proc. SPIE 9657, Industrial Laser Applications Symposium (ILAS 2015), 96570L (1 July 2015); doi: 10.1117/12.2182432
Show Author Affiliations
D. G. Waugh, Univ. of Chester (United Kingdom)
J. Lawrence, Univ. of Chester (United Kingdom)
P. Shukla, Univ. of Chester (United Kingdom)
C. Chan, Queen's Univ. Belfast (United Kingdom)
I. Hussain, Univ. of Lincoln (United Kingdom)
H. C. Man, The Hong Kong Polytechnic Univ. (Hong Kong, China)
G. C. Smith, Univ. of Chester (United Kingdom)

Published in SPIE Proceedings Vol. 9657:
Industrial Laser Applications Symposium (ILAS 2015)
Mike Green; Cath Rose, Editor(s)

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