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Proceedings Paper

Raman spectroscopic analysis of oral squamous cell carcinoma and oral dysplasia in the high-wavenumber region
Author(s): Luis Felipe C. S. Carvalho; Franck Bonnier; Kate O'Callaghan; Jeff O'Sullivan; Stephen Flint; Lazaro P. M. Neto; Cláudio A. T. Soto; Laurita dos Santos; Airton A. Martin; Hugh J. Byrne; Fiona M. Lyng
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Paper Abstract

Raman spectroscopy can provide a molecular-level signature of the biochemical composition and structure of cells with excellent spatial resolution and could be useful to monitor changes in composition for early stage and non-invasive cancer diagnosis, both ex-vivo and in vivo. In particular, the fingerprint spectral region (400–1,800 cm-1) has been shown to be very promising for optical biopsy purposes. However, limitations to discrimination of dysplastic and inflammatory processes based on the fingerprint region still persist. In addition, the Raman spectral signal of dysplastic cells is one important source of misdiagnosis of normal versus pathological tissues. The high wavenumber region (2,800–3,600 cm-1) provides more specific information based on N-H, O-H and C-H vibrations and can be used to identify the subtle changes which could be important for discrimination of samples. In this study, we demonstrate the potential of the highwavenumber spectral region by collecting Raman spectra of nucleoli, nucleus and cytoplasm from oral epithelial cancer (SCC-4) and dysplastic (DOK) cell lines and from normal oral epithelial primary cells, in vitro, which were then analyzed by area under the curve as a method to discriminate the spectra. In this region, we will show the discriminatory potential of the CH vibrational modes of nucleic acids, proteins and lipids. This technique demonstrated more efficient discrimination than the fingerprint region when we compared the cell cultures.

Paper Details

Date Published: 19 June 2015
PDF: 7 pages
Proc. SPIE 9531, Biophotonics South America, 953125 (19 June 2015); doi: 10.1117/12.2180996
Show Author Affiliations
Luis Felipe C. S. Carvalho, Dublin Institute of Technology (Ireland)
Lab. of Biomedical Vibrational Spectroscopy, Univ. do Vale do Paraíba (Brazil)
Franck Bonnier, Dublin Institute of Technology (Ireland)
Kate O'Callaghan, Dublin Dental School and Hospital, Trinity College Dublin (Ireland)
Jeff O'Sullivan, Dublin Dental School and Hospital, Trinity College Dublin (Ireland)
Stephen Flint, Dublin Dental School and Hospital, Trinity College Dublin (Ireland)
Lazaro P. M. Neto, Lab. of Biomedical Vibrational Spectroscopy, Univ. do Vale do Paraíba (Brazil)
Cláudio A. T. Soto, Lab. of Biomedical Vibrational Spectroscopy, Univ. do Vale do Paraíba (Brazil)
Laurita dos Santos, Lab. of Biomedical Vibrational Spectroscopy, Univ. do Vale do Paraíba (Brazil)
Airton A. Martin, Lab. of Biomedical Vibrational Spectroscopy, Univ. do Vale do Paraíba (Brazil)
Hugh J. Byrne, Dublin Institute of Technology (Ireland)
Fiona M. Lyng, Dublin Institute of Technology (Ireland)
Dublin Institute of Technology (Ireland)


Published in SPIE Proceedings Vol. 9531:
Biophotonics South America
Cristina Kurachi; Katarina Svanberg; Bruce J. Tromberg; Vanderlei Salvador Bagnato, Editor(s)

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