Share Email Print
cover

Proceedings Paper

Monolayer to MTS: using SEM, HIM, TEM and SERS to compare morphology, nanosensor uptake and redox potential in MCF7 cells
Author(s): L. E. Jamieson; A. P. Bell; D. J. Harrison; C. J. Campbell
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Cellular redox potential is important for the control and regulation of a vast number of processes occurring in cells. When the fine redox potential balance within cells is disturbed it can have serious consequences such as the initiation or progression of disease. It is thought that a redox gradient develops in cancer tumours where the peripheral regions are well oxygenated and internal regions, further from vascular blood supply, become starved of oxygen and hypoxic. This makes treatment of these areas more challenging as, for example, radiotherapy relies on the presence of oxygen. Currently techniques for quantitative analysis of redox gradients are limited. Surface enhanced Raman scattering (SERS) nanosensors (NS) have been used to detect redox potential in a quantitative manner in monolayer cultured cells with many advantages over other techniques. This technique has considerable potential for use in multicellular tumour spheroids (MTS) – a three dimensional (3D) cell model which better mimics the tumour environment and gradients that develop. MTS are a more realistic model of the in vivo cellular morphology and environment and are becoming an increasingly popular in vitro model, replacing traditional monolayer culture. Imaging techniques such as transmission electron microscopy (TEM), scanning electron microscopy (SEM) and helium ion microscopy (HIM) were used to investigate differences in morphology and NS uptake in monolayer culture compared to MTS. After confirming NS uptake, the first SERS measurements revealing quantitative information on redox potential in MTS were performed.

Paper Details

Date Published: 19 June 2015
PDF: 10 pages
Proc. SPIE 9531, Biophotonics South America, 95311I (19 June 2015); doi: 10.1117/12.2180944
Show Author Affiliations
L. E. Jamieson, Univ. of Edinburgh (United Kingdom)
A. P. Bell, Trinity College (Ireland)
D. J. Harrison, Univ. of St. Andrews (United Kingdom)
C. J. Campbell, Univ. of Edinburgh (United Kingdom)


Published in SPIE Proceedings Vol. 9531:
Biophotonics South America
Cristina Kurachi; Katarina Svanberg; Bruce J. Tromberg; Vanderlei Salvador Bagnato, Editor(s)

© SPIE. Terms of Use
Back to Top