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Proceedings Paper

Optical fluorescence spectroscopy to detect hepatic necrosis after normothermic ischemia: animal model
Author(s): Renan A. Romano; Jose D. Vollet-Filho; Sebastião Pratavieira; Jorge L. Fernandez; Cristina Kurachi; Vanderlei S. Bagnato; Orlando Castro-e-Silva; Ajith K. Sankarankutty
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Paper Abstract

Liver transplantation is a well-established treatment for liver failure. However, the success of the transplantation procedure depends on liver graft conditions. The tissue function evaluation during the several transplantation stages is relevant, in particular during the organ harvesting, when a decision is made concerning the viability of the graft. Optical fluorescence spectroscopy is a good option because it is a noninvasive and fast technique. A partial normothermic hepatic ischemia was performed in rat livers, with a vascular occlusion of both median and left lateral lobes, allowing circulation only for the right lateral lobe and the caudate lobe. Fluorescence spectra under excitation at 532 nm (doubled frequency Nd:YAG laser) were collected using a portable spectrometer (USB2000, Ocean Optics, USA). The fluorescence emission was collected before vascular occlusion, after ischemia, and 24 hours after reperfusion. A morphometric histology analysis was performed as the gold standard evaluation ─ liver samples were analyzed, and the percentage of necrotic tissue was obtained. The results showed that changes in the fluorescence emission after ischemia can be correlated with the amount of necrosis evaluated by a morphometric analysis, the Pearson correlation coefficient of the generated model was 0.90 and the root mean square error was around 20%. In this context, the laser-induced fluorescence spectroscopy technique after normothermic ischemia showed to be a fast and efficient method to differentiate ischemic injury from viable tissues.

Paper Details

Date Published: 19 June 2015
PDF: 8 pages
Proc. SPIE 9531, Biophotonics South America, 95313V (19 June 2015); doi: 10.1117/12.2180897
Show Author Affiliations
Renan A. Romano, Univ. of São Paulo (Brazil)
Jose D. Vollet-Filho, Univ. of São Paulo (Brazil)
Sebastião Pratavieira, Univ. of São Paulo (Brazil)
Jorge L. Fernandez, Medical School of Ribeirão Preto, Univ. of São Paulo (Brazil)
Cristina Kurachi, Univ. of São Paulo (Brazil)
Vanderlei S. Bagnato, Univ. of São Paulo (Brazil)
Orlando Castro-e-Silva, Medical School of Ribeirão Preto, Univ. of São Paulo (Brazil)
Ajith K. Sankarankutty, Medical School of Ribeirão Preto, Univ. of São Paulo (Brazil)


Published in SPIE Proceedings Vol. 9531:
Biophotonics South America
Cristina Kurachi; Katarina Svanberg; Bruce J. Tromberg; Vanderlei Salvador Bagnato, Editor(s)

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