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Proceedings Paper

Fluorescence spectroscopy for assessment of liver transplantation grafts concerning graft viability and patient survival
Author(s): José D. Vollet Filho; Marina R. da Silveira; Orlando Castro-e-Silva; Vanderlei S. Bagnato; Cristina Kurachi
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Paper Abstract

Evaluating transplantation grafts at harvest is essential for its success. Laser-induced fluorescence spectroscopy (LIFS) can help monitoring changes in metabolic/structural conditions of tissue during transplantation. The aim of the present study is to correlate LIFSobtained spectra of human hepatic grafts during liver transplantation with post-operative patients' mortality rate and biochemical parameters, establishing a method to exclude nonviable grafts before implantation. Orthotopic liver transplantation, piggyback technique was performed in 15 patients. LIFS was performed under 408nm excitation. Collection was performed immediately after opening donor’s abdominal cavity, after cold perfusion, end of back-table period, and 5 min and 1 h after warm perfusion at recipient. Fluorescence information was compared to lactate, creatinine, bilirubin and INR levels and to survival status. LIFS was sensitive to liver changes during transplantation stages. Study-in-progress; initial results indicate correlation between fluorescence and life/death status of patients.

Paper Details

Date Published: 19 June 2015
PDF: 6 pages
Proc. SPIE 9531, Biophotonics South America, 95310X (19 June 2015); doi: 10.1117/12.2180730
Show Author Affiliations
José D. Vollet Filho, Univ. de São Paulo (Brazil)
Marina R. da Silveira, Ribeirao Preto Medical School, Univ. de Sao Paulo (Brazil)
Orlando Castro-e-Silva, Ribeirao Preto Medical School, Univ. de Sao Paulo (Brazil)
Vanderlei S. Bagnato, Univ. de São Paulo (Brazil)
Cristina Kurachi, Univ. de São Paulo (Brazil)


Published in SPIE Proceedings Vol. 9531:
Biophotonics South America
Cristina Kurachi; Katarina Svanberg; Bruce J. Tromberg; Vanderlei Salvador Bagnato, Editor(s)

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