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Proceedings Paper

Optical detection of two-color-fluorophore barcode for nanopore DNA sensing
Author(s): M. Zhang; I. Sychugov; T. Schmidt; J. Linnros
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Paper Abstract

A simple schematic on parallel optical detection of two-fluorophore barcode for single-molecule nanopore sensing is presented. The chosen two fluorophores, ATTO-532 and DY-521-XL, emitting in well-separated spectrum range can be excited at the same wavelength. A beam splitter was employed to separate signals from the two fluorophores and guide them to the same CCD camera. Based on a conventional microscope, sources of background in the nanopore sensing system, including membranes, compounds in buffer solution, and a detection cell was characterized. By photoluminescence excitation measurements, it turned out that silicon membrane has a negligible photoluminescence under the examined excitation from 440 nm to 560 nm, in comparison with a silicon nitrite membrane. Further, background signals from the detection cell were suppressed. Brownian motion of 450 bps DNA labelled with single ATTO-532 or DY-521-XL was successfully recorded by our optical system.

Paper Details

Date Published: 1 June 2015
PDF: 7 pages
Proc. SPIE 9519, Nanotechnology VII, 95190G (1 June 2015); doi: 10.1117/12.2179626
Show Author Affiliations
M. Zhang, KTH Royal Institute of Technology (Sweden)
I. Sychugov, KTH Royal Institute of Technology (Sweden)
T. Schmidt, KTH Royal Institute of Technology (Sweden)
J. Linnros, KTH Royal Institute of Technology (Sweden)


Published in SPIE Proceedings Vol. 9519:
Nanotechnology VII
Ion M. Tiginyanu, Editor(s)

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