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Proceedings Paper

Real-time characterization of the neuronal response to osmotic shock by digital holographic microscopy
Author(s): Maria Gomariz; Isabel Garcia; Cristina Soto-Sánchez; Gema Martínez-Navarrete; Eduardo Fernández; Antonio Fimia
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Paper Abstract

Digital holographic microscopy has numerous applications in biology for visualizing living cells and 3D tissues. This technique allows for the direct visualization of biological structures avoiding invasive and phototoxic procedures such as fixation and dying processing. In this study we have characterized the morphometry changes of neurons subject to osmolarity changes. For this purpose, we have measured the variations of the amplitude and the oscillation frequency of the plasmatic membrane, as well as the volume changes of the cells before the osmotic shock. There was a relation between the neural culture ageing and its behavioral changes. "Long-term" cultures that had not previously been studied were used to analyze the behavioral changes in aged cells.

Paper Details

Date Published: 8 May 2015
PDF: 7 pages
Proc. SPIE 9508, Holography: Advances and Modern Trends IV, 950803 (8 May 2015); doi: 10.1117/12.2179116
Show Author Affiliations
Maria Gomariz, Univ. Miguel Hernández de Elche (Spain)
Isabel Garcia, Univ. Miguel Hernández de Elche (Spain)
Cristina Soto-Sánchez, Univ. Miguel Hernández de Elche (Spain)
CIBER-BBN (Spain)
Gema Martínez-Navarrete, Univ. Miguel Hernández de Elche (Spain)
Eduardo Fernández, Univ. Miguel Hernández de Elche (Spain)
CIBER-BBN (Spain)
Antonio Fimia, Univ. Miguel Hernández de Elche (Spain)
CIBER-BBN (Spain)


Published in SPIE Proceedings Vol. 9508:
Holography: Advances and Modern Trends IV
Miroslav Hrabovský; John T. Sheridan; Antonio Fimia, Editor(s)

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