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Proceedings Paper

Quenching of photoexcited states of the proteins chromophores and introduced into the protein macromolecules fluorescent probes by heavy metal ions
Author(s): A. G. Melnikov; O. A. Dyachuk; G. V. Melnikov
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Paper Abstract

We have studied the processes of quenching of photoexcited states of fluorescent probes and quenching of the fluorescence of the chromophores of human serum albumin (HSA) by heavy metal ions (HM): cations Tl+, Pb2+, Cu2+, Cd2+, and the anion of iodine (I-). We used the dye from xanthene series – eosin as a fluorescent probe. By quenching of the fluorescence of protein chromophores we found an influence of HM on the structure of proteins, resulting in a shift of the peak of the fluorescence of HSA tryptophanyl. This can be explained by proteins denaturation under the influence of heavy metals and penetration of water into the inner environment of HSA tryptophan. It was established that the constant of the quenching of the probe phosphorescence is much higher than the fluorescence, which is explained by significantly longer lifetime of the photoexcited states of fluorescent probes in the triplet state than in the singlet.

Paper Details

Date Published: 19 March 2015
PDF: 7 pages
Proc. SPIE 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics, 94480S (19 March 2015); doi: 10.1117/12.2178372
Show Author Affiliations
A. G. Melnikov, Yuri Gagarin State Technical Univ. of Saratov (Russian Federation)
O. A. Dyachuk, Yuri Gagarin State Technical Univ. of Saratov (Russian Federation)
G. V. Melnikov, Yuri Gagarin State Technical Univ. of Saratov (Russian Federation)


Published in SPIE Proceedings Vol. 9448:
Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics
Elina A. Genina; Vladimir L. Derbov; Kirill V. Larin; Dmitry E. Postnov; Valery V. Tuchin, Editor(s)

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