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Proceedings Paper

Antimicrobial blue light therapy for Candida albicans burn infection in mice
Author(s): Yunsong Zhang; Yucheng Wang; Clinton K. Murray; Michael R. Hamblin; Ying Gu; Tianhong Dai
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Paper Abstract

In this preclinical study, we investigated the utility of antimicrobial blue light therapy for Candida albicans infection in acutely burned mice. A bioluminescent strain of C. albicans was used. The susceptibilities to blue light inactivation were compared between C. albicans and human keratinocyte. In vitro serial passaging of C. albicans on blue light exposure was performed to evaluate the potential development of resistance to blue light inactivation. A mouse model of acute thermal burn injury infected with the bioluminescent strain of C. albicans was developed. Blue light (415 nm) was delivered to mouse burns for decolonization of C. albicans. Bioluminescence imaging was used to monitor in real time the extent of fungal infection in mouse burns. Experimental results showed that C. albicans was approximately 42-fold more susceptible to blue light inactivation in vitro than human keratinocyte (P=0.0022). Serial passaging of C. albicans on blue light exposure implied a tendency for the fungal susceptibility to blue light inactivation to decrease with the numbers of passages. Blue light reduced fungal burden by over 4-log10 (99.99%) in acute mouse burns infected with C. albicans in comparison to infected mouse burns without blue light therapy (P=0.015).

Paper Details

Date Published: 22 May 2015
PDF: 13 pages
Proc. SPIE 9467, Micro- and Nanotechnology Sensors, Systems, and Applications VII, 94670K (22 May 2015); doi: 10.1117/12.2178232
Show Author Affiliations
Yunsong Zhang, Jinan Univ. (China)
Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Yucheng Wang, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Chinese PLA General Hospital (China)
Clinton K. Murray, Brooke Army Medical Ctr. (United States)
Michael R. Hamblin, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Ying Gu, Chinese PLA General Hospital (China)
Tianhong Dai, Massachusetts General Hospital (United States)
Harvard Medical School (United States)


Published in SPIE Proceedings Vol. 9467:
Micro- and Nanotechnology Sensors, Systems, and Applications VII
Thomas George; Achyut K. Dutta; M. Saif Islam, Editor(s)

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