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Proceedings Paper

A silicon-based peptide biosensor for label-free detection of cancer cells
Author(s): Nicola M. Martucci; Ilaria Rea; Immacolata Ruggiero; Monica Terracciano; Luca De Stefano; Nunzia Migliaccio; Principia Dardano; Paolo Arcari; Ivo Rendina; Annalisa Lamberti
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Paper Abstract

Sensitive and accurate detection of cancer cells plays a crucial role in diagnosis of cancer and minimal residual disease, so being one of the most hopeful approaches to reduce cancer death rates. In this paper, a strategy for highly selective and sensitive detection of lymphoma cells on planar silicon-based biosensor has been evaluated. In this setting an Idiotype peptide, able to specifically bind the B-cell receptor (BCR) of A20 cells in mice engrafted with A20 lymphoma, has been covalently linked to the sensor active surface and used as molecular probe. The biochip here presented showed a coverage efficiency of 85% with a detection efficiency of 8.5×10-3 cells/μm2. The results obtained suggested an efficient way for specific label-free cell detection by using a silicon-based peptide biosensor. In addition, the present recognition strategy, besides being useful for the development of sensing devices capable of monitoring minimal residual disease, could be used to find and characterize new specific receptor-ligand interactions through the screening of a recombinant phage library.

Paper Details

Date Published: 5 May 2015
PDF: 8 pages
Proc. SPIE 9506, Optical Sensors 2015, 95061T (5 May 2015); doi: 10.1117/12.2178137
Show Author Affiliations
Nicola M. Martucci, Univ. degli Studi di Napoli Federico II (Italy)
Ilaria Rea, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
Immacolata Ruggiero, Univ. degli Studi di Napoli Federico II (Italy)
Monica Terracciano, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
Univ. degli Studi di Naples Frederico II (Italy)
Luca De Stefano, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
Nunzia Migliaccio, Univ. degli Studi di Napoli Federico II (Italy)
Principia Dardano, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
Paolo Arcari, Univ. degli Studi di Napoli Federico II (Italy)
Ivo Rendina, Istituto per la Microelettronica e Microsistemi (Italy)
Annalisa Lamberti, Univ. degli Studi di Napoli Federico II (Italy)


Published in SPIE Proceedings Vol. 9506:
Optical Sensors 2015
Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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