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Proceedings Paper

Snapshot imaging Fraunhofer line discriminator for detection of plant fluorescence
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Paper Abstract

Non-invasive quantification of plant health is traditionally accomplished using reflectance based metrics, such as the normalized difference vegetative index (NDVI). However, measuring plant fluorescence (both active and passive) to determine photochemistry of plants has gained importance. Due to better cost efficiency, lower power requirements, and simpler scanning synchronization, detecting passive fluorescence is preferred over active fluorescence. In this paper, we propose a high speed imaging approach for measuring passive plant fluorescence, within the hydrogen alpha Fraunhofer line at ~656 nm, using a Snapshot Imaging Fraunhofer Line Discriminator (SIFOLD). For the first time, the advantage of snapshot imaging for high throughput Fraunhofer Line Discrimination (FLD) is cultivated by our system, which is based on a multiple-image Fourier transform spectrometer and a spatial heterodyne interferometer (SHI). The SHI is a Sagnac interferometer, which is dispersion compensated using blazed diffraction gratings. We present data and techniques for calibrating the SIFOLD to any particular wavelength. This technique can be applied to quantify plant fluorescence at low cost and reduced complexity of data collection.

Paper Details

Date Published: 21 May 2015
PDF: 9 pages
Proc. SPIE 9472, Algorithms and Technologies for Multispectral, Hyperspectral, and Ultraspectral Imagery XXI, 94721J (21 May 2015); doi: 10.1117/12.2176906
Show Author Affiliations
S. Gupta Roy, North Carolina State Univ. (United States)
M. W. Kudenov, North Carolina State Univ. (United States)


Published in SPIE Proceedings Vol. 9472:
Algorithms and Technologies for Multispectral, Hyperspectral, and Ultraspectral Imagery XXI
Miguel Velez-Reyes; Fred A. Kruse, Editor(s)

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