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Proceedings Paper

Reproducible and time-course study of yeast biofilm by Raman spectroscopy
Author(s): Ota Samek; Katarina Mlynariková; Silvie Bernatová; Jan Ježek; Vladislav Kryžánek; Martin Šiler; Pavel Zemánek; Filip Růžička
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Paper Abstract

We report on Raman spectroscopy measurements - separated by a given time intervals - for the selected yeast strains (biofilm positive and biofilm negative) on colonies grown directly on the Petri dishes or on the well-plate. Chemometric principal component analysis of these spectra sets generated clusters of data points, from which the reproducibility of the measurement could be analysed. Consequently, these resulted in clusters coinciding well with the biofilm positive and biofilm negative strains measurement of a particular sample dish, suggesting good reproducibility of our measurement procedure, even when the samples were prepared and measured days up to months apart. This suggests the potential of Raman spectroscopy in routine clinical diagnostic.

Paper Details

Date Published: 5 December 2014
PDF: 9 pages
Proc. SPIE 9441, 19th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 94411M (5 December 2014); doi: 10.1117/12.2176031
Show Author Affiliations
Ota Samek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Katarina Mlynariková, Faculty of Medicine and St. Anne's Univ. Hospital (Czech Republic)
Silvie Bernatová, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Jan Ježek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Vladislav Kryžánek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Martin Šiler, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Pavel Zemánek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Filip Růžička, Faculty of Medicine and St. Anne's Univ. Hospital (Czech Republic)


Published in SPIE Proceedings Vol. 9441:
19th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics
Agnieszka Popiolek-Masajada; Waclaw Urbanczyk, Editor(s)

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