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Proceedings Paper

Diagnosis of mitochondrial diseases by near-infrared spectroscopy (NIRS)
Author(s): William J. Bank; Britton Chance
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Paper Abstract

Disorders of mitochondrial metabolism are manifest by inordinate fatigue, weakness, as well as severe neuromuscular disorders. Diagnosis has required pathologic findings on muscle biopsy and identification of biochemical defects in mitochondrial respiration. NIRS, a noninvasive optical technique, permits the quantitative measurement of changes in blood volume and tissue oxygenation in vivo, at rest, during exercise, and post-exercise recovery. The dual wavelength spectrophotometer consists of an optic probe with 2 lights appropriate for red light emission. Interference filters select the wavelengths, 760 to 850 nm, appropriate to the broad bands of hemoglobin, in conjunction with silicon detectors sensitive to this infrared spectrum. In all normal test subjects, the blood volume tracing demonstrated a decreased blood volume normally seen in exercising muscle. The increase of absorbance at 760 nm, with respect to absorbance at 850 nm, reflects deoxygenation of hemoglobin and occurred promptly at the start of exercise. At the end of exercise, oxygenation returned to baseline accompanied by hyperemia. Four patients with known disorders of mitochondrial metabolism demonstrated a paradoxical oxygenation during exercise that returned to baseline at the end of exercise. Increased oxygen supplied by a normal cardiopulmonary response to exercise is not utilized and results in a pardoxical oxygenation during exercise. This simple, noninvasive technique permits an accurate measurement of oxygen utilization in the exercising limb and is a useful clinical tool in screening patients for disorders of mitochondrial metabolism.

Paper Details

Date Published: 30 May 1995
PDF: 6 pages
Proc. SPIE 2389, Optical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media: Theory, Human Studies, and Instrumentation, (30 May 1995); doi: 10.1117/12.210026
Show Author Affiliations
William J. Bank, Univ. of Pennsylvania (United States)
Britton Chance, Univ. of Pennsylvania (United States)

Published in SPIE Proceedings Vol. 2389:
Optical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media: Theory, Human Studies, and Instrumentation
Britton Chance; Robert R. Alfano, Editor(s)

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