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Proceedings Paper

Fluorescence lifetime spectroscopy and imaging in random media
Author(s): Christina L. Hutchinson; Tamara L. Troy; Eva Marie Sevick-Muraca
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Paper Abstract

Tissue fluorescence, whether from endogenous or exogenous probes, provides an opportunity for interrogation on the basis of structure and function. However to date, there has been little understanding of the localization of signal origin of fluorescence signals re-emitted from tissues. Previously, we have shown using finite element computations that the origin or re- emitted fluorescence signal depends upon the lifetime of the optical probe. The signal arising from long-lived phosphorescent probes may predominately come from the tissue-air interface rather than from deep within the tissue. Therefore, the use of short-lived optical probes may be desirable for fluorescence spectroscopy and as contrast agents for biomedical optical lived probes embedded within scattering media. Our results show that upon proper referencing of frequency-domain measurements, lifetime measurements can be made in the presence of uniform and nonuniform distribution of optical probes. The implications for biomedical optical imaging on the basis of probe lifetime are discussed.

Paper Details

Date Published: 30 May 1995
PDF: 10 pages
Proc. SPIE 2389, Optical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media: Theory, Human Studies, and Instrumentation, (30 May 1995); doi: 10.1117/12.209977
Show Author Affiliations
Christina L. Hutchinson, Purdue Univ. (United States)
Tamara L. Troy, Purdue Univ. (United States)
Eva Marie Sevick-Muraca, Purdue Univ. (United States)


Published in SPIE Proceedings Vol. 2389:
Optical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media: Theory, Human Studies, and Instrumentation
Britton Chance; Robert R. Alfano, Editor(s)

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