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Proceedings Paper

Adenylate pool and energy charge in human lymphocytes and granulocytes irradiated at 632 nm (HeNe laser)
Author(s): Lorenzo Bolognani; T. Venturelli; N. Volpi; O. Zirilli
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Paper Abstract

Aim of this report was to investigate the adenylate pool and the energy charge in human white blood cells exposed to increasing time (15, 30 and 60 min) of HeNe laser treatment. EDTA treated human blood diluted 1:1 with 0.88% KCl was added (1:5) with NaCl-dextran solution to allow sedimentation of red blood cells. 6 ml of the white cells floating in the supernatant were layered on 3 ml of Lymphoprep in plastic tubes and each tube was centrifuged (from 50 to 5000 X g for 5 min). Granulocytes were concentrated in the lower phase, whilst lymphocytes were in the intermediated phase. After further purification cytological homogeneity was tested by a cell counter. Granulocytes and lymphocytes were irradiated at +22°C with HeNe (Space, Valfivre equipment). On these population ATP was tested by luminometric procedure, the adenylate pool was separated by HPLC (Jasco) on neutralyzed perchloric extracts. ATP concentration increased in lymphocytes (+63.9%, p < 0.01) and in granulocytes (+25.0%, p < 0.05) after 60 min irradiation. The adenylate pool (tested by HPLC) does not change significatively in lymphocytes or granulocytes after 30 min irradiation, whilst in 60 min irradiated lymphocytes and granulocytes a significative increment was observed in nucleotide concentration. No changes were observed in energy charge according to Atkinson.

Paper Details

Date Published: 22 May 1995
PDF: 7 pages
Proc. SPIE 2391, Laser-Tissue Interaction VI, (22 May 1995); doi: 10.1117/12.209928
Show Author Affiliations
Lorenzo Bolognani, Univ. degli Studi di Modena (Italy)
T. Venturelli, Univ. degli Studi di Modena (Italy)
N. Volpi, Univ. degli Studi di Modena (Italy)
O. Zirilli, Univ. degli Studi di Modena (Italy)

Published in SPIE Proceedings Vol. 2391:
Laser-Tissue Interaction VI
Steven L. Jacques, Editor(s)

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