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Proceedings Paper

Does low intensity He-Ne laser radiation affect the intracellular pH of intact Escherichia coli cells?
Author(s): Terence I. Quickenden; Lillian L. L. Daniels; Lyndsay T. Byrne
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Paper Abstract

Claims that low levels of He-Ne laser light (cw, (lambda) equals 632.8 nm) can provide clinical benefits and can enhance in vitro cellular growth are still controversial (T.I. Quickenden and L.L. Daniels, 1993, Photochem. Photobiol. 57, 272-278; L.L. Daniels and T.I. Quickenden, 1994, Photochem. Photobiol., 60, 481-485). The present study tests the suggestion (T.I. Karu, 1988, Lasers life Sci. 2, 53-74; H. Friedmann, R. Lubart, I. Laulicht and S. Rochkink, 1991, J. Photochem. Photobiol. B: Biol. 11, 87-91) that red light stimulates mitosis by raising intracellular pH via absorption by chromophores in the respiratory chain. In order to search for photoinduced changes in intracellular pH, the effect of 5 mW He-Ne laser irradiation on cultures of E. coli was examined using a 300 MHz Nuclear Magnetic Resonance (NMR) spectrometer. The pH difference between the intracellular and extracellular fluid was monitored in the presence and absence of radiation by determining the difference in chemical shift for 31P resonances arising from the H2PO4- ⇔ HPO42- + H+ equilibrium in the two environments.

Paper Details

Date Published: 22 May 1995
PDF: 11 pages
Proc. SPIE 2391, Laser-Tissue Interaction VI, (22 May 1995); doi: 10.1117/12.209924
Show Author Affiliations
Terence I. Quickenden, Univ. of Western Australia (Australia)
Lillian L. L. Daniels, Univ. of Western Australia (Australia)
Lyndsay T. Byrne, Univ. of Western Australia (Australia)


Published in SPIE Proceedings Vol. 2391:
Laser-Tissue Interaction VI
Steven L. Jacques, Editor(s)

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