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Proceedings Paper

Microthermometry of laser-heated Chinese hamster ovary cells and sperm cells
Author(s): Yagang Liu; Gregory J. Sonek; Curtis F. Chapman; Bruce J. Tromberg; Pasquale Patrizio; Yona Tadir; Michael W. Berns
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Paper Abstract

Microthermometric measurements on optically-trapped Chinese Hamster Ovary (CHO) cells and sperms cells re reported, using a noninvasive microfluorometric detection technique. Within an optical tweezer system that has been outfitted with a spectral fluorescence excitation and detection capability, the changes in temperature induced by the process of sample confinement by a focused laser beam has been quantified over micron-sized spatial regions of both motile and immotile cells. Our measurement technique is based on the use of environmentally sensitive fluorophores that can be incorporated into the cell membrane and used to sense local changes in temperature when the cell membrane is perturbed optically or via other environmental stress factors. Using a cw 1.064 micrometers Nd:YAG laser for trapping CHO and human sperm cells, a temperature increase of approximately equals 1°C per 100 mW laser power was observed. At this infrared wavelength, cellular heating as result of laser confinement appears to be mainly due to radiation absorption by water.

Paper Details

Date Published: 22 May 1995
PDF: 7 pages
Proc. SPIE 2391, Laser-Tissue Interaction VI, (22 May 1995); doi: 10.1117/12.209917
Show Author Affiliations
Yagang Liu, Univ. of California/Irvine and Beckman Laser Institute and Medical Clinic (United States)
Gregory J. Sonek, Univ. of California/Irvine and Beckman Laser Institute and Medical Clinic (United States)
Curtis F. Chapman, Beckman Laser Institute and Medical Clinic (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)
Pasquale Patrizio, Univ. of California/Irvine (United States)
Yona Tadir, Beckman Laser Institute and Medical Clinic (United States)
Michael W. Berns, Beckman Laser Institute and Medical Clinic (United States)


Published in SPIE Proceedings Vol. 2391:
Laser-Tissue Interaction VI
Steven L. Jacques, Editor(s)

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