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Proceedings Paper

Melanin may promote photooxidation of linoleic acid
Author(s): Randolph D. Glickman; Kwok-Wai Lam
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Paper Abstract

We have previously shown that laser-exposed melanin granules isolated from the retinal pigment epithelium (RPE) are capable of oxidizing ascorbic acid. We are now characterizing the reactions of light- activated melanin with other cellular components such as linoleic acid, a polyunsaturated fatty acid. Commercial linoleic acid, and melanin granules isolated from bovine RPE cells, are mixed and exposed to the broad band output of a 150 W Xenon arc lamp or the CW output of an Argon laser. Native linoleic acid is separated from its hydroperoxides by HPLC, and the relative amounts of each are detected by UV absorbance at 210 and 232 nm, respectively. Exposure of the linoleic acid alone to the xenon arc source results in production of linoleic hydroperoxides (LHP) in an intensity-dependent reaction that doubles in extent over the temperature range of 0° to 80°C. Addition of melanin granules at a density of 108 granules/ml reduces the production of LHP, probably because of light absorption and self-screening by the melanin. At or below a density of 107 granules/ml, however, the light-driven production of LHP is enhanced, especially during exposure to the blue- green output of the Argon laser. Physiological antioxidants (Vit. C,E protect the linoleic acid from photo-oxidation in the presence or absence of melanin. These observations support the hypothesis that light-activated melanin can react with some cellular components and thereby contribute to photochemical damage, especially if endogenous antioxidants are depleted.

Paper Details

Date Published: 22 May 1995
PDF: 8 pages
Proc. SPIE 2391, Laser-Tissue Interaction VI, (22 May 1995); doi: 10.1117/12.209890
Show Author Affiliations
Randolph D. Glickman, Univ. of Texas Health Science Ctr./San Antonio (United States)
Kwok-Wai Lam, Univ. of Texas Health Science Ctr./San Antonio (United States)

Published in SPIE Proceedings Vol. 2391:
Laser-Tissue Interaction VI
Steven L. Jacques, Editor(s)

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