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Proceedings Paper

Corneal wound healing after photoablation with a 213-nm scanning solid state laser: histological and ultrastructural study
Author(s): Jean-Marc Legeais; Jean-Marie A. Parel; Michele Savoldelli; Isabelle Drubaix; Per G. Soederberg; Fabrice Manns; Gilles Renard; Yves Pouliquen
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Paper Abstract

To investigate wound healing in rabbits after corneal photoablation with a 213 nm UV scanning solid state laser. We used a frequency quintupled Nd:YAG laser to photoablate the cornea of 32 rabbits (5 mm ablation zone, 6 D myopic correction). The contralateral eyes and the eyes of 3 untreated animals served as controls. Light and electron microscopy analyses were performed on postoperative days 0, 7, 28, 90. Abnormal, light basal epithelial cells were observed during the first postoperative month, but normal epithelial maturation was evident at 3 months. Basement membrane duplication was noted. Keratocyte proliferation occurred in the anterior stroma and in a few cases, in the posterior stroma, where keratocyte activation was present. Degenerative endothelial changes were observed immediately after ablation with intracellular disorganization and junction alteration. Surface profiles with variable regularity led to a variable wound healing. Apart from differences in epithelial basal cell appearance, photoablation with a 213 nm solid state laser induced wound healing processes similar to those observed previously with prototype ArF excimer lasers. It is hoped that laser refinements will improve the surface regularity and lead to more consistent wound healing responses. However, several studies are required to assess mutagenicity, penetration depth and ablation rate of the 213 nm wavelength on ocular tissues, as well as the effects of hydration on the clinical outcome.

Paper Details

Date Published: 22 May 1995
PDF: 8 pages
Proc. SPIE 2393, Ophthalmic Technologies V, (22 May 1995); doi: 10.1117/12.209857
Show Author Affiliations
Jean-Marc Legeais, Univ. de Paris Hopital de l'Hotel-Dieu (France)
Jean-Marie A. Parel, Univ. de Paris Hopital de l'Hotel-Dieu (France)
Bascom Palmer Eye Institute/Univ. of Miami School of Medicine (United States)
Michele Savoldelli, Univ. de Paris Hopital de l'Hotel-Dieu (France)
Isabelle Drubaix, Univ. de Paris Hopital de l'Hotel-Dieu (France)
Per G. Soederberg, Bascom Palmer Eye Institute/Univ. of Miami School of Medicine (United States)
Fabrice Manns, Bascom Palmer Eye Institute/Univ. of Miami School of Medicine (United States)
Gilles Renard, Univ. de Paris Hopital de l'Hotel-Dieu (France)
Yves Pouliquen, Univ. de Paris Hopital de l'Hotel-Dieu (France)


Published in SPIE Proceedings Vol. 2393:
Ophthalmic Technologies V
Jean-Marie Parel; Qiushi Ren; Karen Margaret Joos, Editor(s)

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